Effect of culture conditions on Escherichia coli O157:H7-mediated attaching-effacing lesions in a bovine large intestinal mucosal explant model

Angela A. Baehler, Rodney A. Moxley

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The effects of culture conditions on the extent of Escherichia coli O157:H7 attaching-effacing (A/E) adherence in an adult bovine large intestinal mucosal explant model were assessed by three different morphometric methods. Measurement of the percent of tissue sections with A/E adherence and the number of foci of A/E adherence mm-1 of surface epithelium was more sensitive than measurement of the percent of surface epithelium with A/E adherent bacteria for detection of treatment effects. Culture of bacterial inoculum in tryptic soy broth, incubation of explants in 5% CO2, and rocking of explants on a platform rocker at 18 cycles min-1 provided optimal conditions for A/E adherence. In future studies, the model may be used for preliminary testing of intervention strategies aimed at reduction of E. coli O157:H7 intestinal colonization of cattle.

Original languageEnglish (US)
Pages (from-to)107-110
Number of pages4
JournalFEMS Microbiology Letters
Volume212
Issue number1
DOIs
StatePublished - Jun 18 2002

Keywords

  • Attaching-effacing
  • Cattle
  • Escherichia coli O157:H7
  • Explant
  • Large intestine
  • Morphometry

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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