Effect of diluent chain length on the performance of the electrochemical DNA sensor at elevated temperature

Weiwei Yang, Rebecca Y. Lai

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Here we report the effect of passivating diluent chain length and sensor interrogation temperature on the electrochemical DNA (E-DNA) sensor's mismatch discrimination capability. Both stem-loop and linear probe-based E-DNA sensors were constructed with various diluents, including 6-mercapto-1-hexanol and longer chain hydroxyl-terminated alkanethiols. Contrary to previously reported results, we find that the E-DNA sensors work optimally in the presence of the longer chain diluents, signified by the enhanced % signal suppression observed upon target hybridization. Of note, the sensors' signaling efficiency maintains even when interrogated at an elevated temperature, permitting the use of stringent temperature conditions to improve sensor specificity. For example, a stem-loop E-DNA sensor fabricated with 8-mercapto-1-octanol, when employed at 47 °C, produces signal suppression of 79%, 35% and 1.6% for the perfect match, single-base mismatch, and 2-base mismatch DNA targets, respectively. In addition to the significant enhancement in sensor discrimination capacity, high temperature operation also improves hybridization kinetics. Our results also suggest that the stem-loop E-DNA sensors demonstrate better mismatch discrimination capability when compared to the linear probe system under the same experimental condition.

Original languageEnglish (US)
Pages (from-to)134-139
Number of pages6
JournalAnalyst
Volume136
Issue number1
DOIs
StatePublished - Jan 7 2011

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Fingerprint Dive into the research topics of 'Effect of diluent chain length on the performance of the electrochemical DNA sensor at elevated temperature'. Together they form a unique fingerprint.

  • Cite this