Effect of ethanol on uptake of choline by the isolated perfused rat liver

D. J. Tuma, R. C. Keeper, H. C. Beckenhauer, A. J. Barak

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

1. 1. Insofar as most studies on the effects of ethanol on the organism have involved chronic ingestion, this study was designed to measure the acute influence of this alcohol on choline uptake in the liver. 2. 2. Using the isolated perfused liver technique it was found that ethanol circulated in the perfusate increased the uptake of exogenous choline by the perfused liver. Acetate supplements had no effect on the uptake. 3. 3. It was found that increased uptake was not unique to ethanol and that two other alcohols that are NADH generators, methanol and sorbitol, also increased choline uptake. 4. 4. A hydrogen acceptor, 2,3,5-triphenyltetrazolium chloride, was shown to depress choline uptake. This finding tends to implicate NADH in the mechanism responsible for increased choline uptake seen in this study. 5. 5. It is concluded from this study that of the acetate and NADH resulting from ethanol metabolism, NADH is possibly the most potent stimulator of choline uptake.

Original languageEnglish (US)
Pages (from-to)141-147
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume218
Issue number1
DOIs
StatePublished - Oct 6 1970

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

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