TY - JOUR
T1 - Effect of growth in serum on uptake of neisseria gonorrhoeae by human neutrophils
AU - Britigan, Bradley
AU - Cohen, Myron S.
N1 - Funding Information:
Received for publication October to, 1984,and in revised form February 19, 1985. These studies were supported by grants AI-15036and AI-07151-06 from the National Institutes of Health. Dr. Cohen is the recipient of the R. J. ReynoldsAward and the Jefferson-Pilot Award. Wethank Marjorie Cooney for technical assistance; P. Frederick Sparling for suggestions and criticisms; Janne Cannon, Rick Schwalbe, and John Black for help with outer membrane protein analysis; and Diane Smith and Belinda Wilson for assistance in manuscript preparation. Please address requests for reprints to Dr. Myron S. Cohen, Department of Medicine, University of North Carolina at Chapel Hill, 547 Burnett-Womack Clinical Sciences Building 229H, Chapel Hill, North Carolina 27514
PY - 1985/8/1
Y1 - 1985/8/1
N2 - Because serum exposure precedes interaction of invasive Neisseria gonorrhoeae with neutrophils, neutrophil association with gonococci grown in serum was assessed. Uptake of a nonpiliated serum-resistant strain grown in 20% serum was reduced to 63.5% of control values. Heated serum (20%) yielded similar results. Stimulation of hexose monophosphate shunt activity in neutrophils by heated serum-grown gonococci (a phenomenon reflecting phagocytosis) was 64.5% of control values. Because gonococcal outer membrane (OM) structures mediate interaction with neutrophils, lithium acetate-treated OM preparations of gonococci grown in heated serum were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Multiple new protein bands and a quantitative decrease in amounts of proteins I, II, and III were noted. However, a decrease in the amounts of these membrane proteins was not observed with alternative membrane extraction techniques. Gonococcal growth in 20% serum dialyzed in 3, 500-molecular-weight exclusion tubing allowed normal neutrophil association and OM protein expression.
AB - Because serum exposure precedes interaction of invasive Neisseria gonorrhoeae with neutrophils, neutrophil association with gonococci grown in serum was assessed. Uptake of a nonpiliated serum-resistant strain grown in 20% serum was reduced to 63.5% of control values. Heated serum (20%) yielded similar results. Stimulation of hexose monophosphate shunt activity in neutrophils by heated serum-grown gonococci (a phenomenon reflecting phagocytosis) was 64.5% of control values. Because gonococcal outer membrane (OM) structures mediate interaction with neutrophils, lithium acetate-treated OM preparations of gonococci grown in heated serum were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Multiple new protein bands and a quantitative decrease in amounts of proteins I, II, and III were noted. However, a decrease in the amounts of these membrane proteins was not observed with alternative membrane extraction techniques. Gonococcal growth in 20% serum dialyzed in 3, 500-molecular-weight exclusion tubing allowed normal neutrophil association and OM protein expression.
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U2 - 10.1093/infdis/152.2.330
DO - 10.1093/infdis/152.2.330
M3 - Article
C2 - 3928768
AN - SCOPUS:0021877444
SN - 0022-1899
VL - 152
SP - 330
EP - 338
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -