Effect of human immunodeficiency virus type 1 (HIV-1) nucleocapsid protein on HIV-1 reverse transcriptase activity in vitro

Xiaodong Ji, George J. Klarmann, Bradley D. Preston

Research output: Contribution to journalArticlepeer-review

116 Scopus citations


Asp537 and Asp812 are essential in the catalytic mechanism of T7 RNA polymerase. The mutants D537N and D812N have no detectable activity whereas the mutants D537E and D812E have significantly reduced activity relative to the wild-type. The hypothesis that these two amino acids act as metal- binding ligands has been tested using EPR with Mn2+ as the metal ion. Mn2+ is able to substitute for Mg2+ in transcription by T7 RNAP on templates containing the T7 promoter. Mg2+ and Mn2+ compete for binding sites, with the former having lower affinity. Mn2+ binding to the wild- type enzyme and the mutants D537N, D812N, D537E, D812E, and Y649F was measured over the concentration range of 25 μM 1.5 mM. The data were analyzed by nonlinear least-squares fits to the binding isotherms, and the analysis gave approximately two Mn2+-binding sites in all cases and a K(d) for the wild-type of ~340 μM. The K(d) value for the mutant Y639F, in which Asp537 and Asp 812 are not mutated, is comparable to the value for the wild- type. Mn2+ binding to the double mutants, D537N/D812N and D537E/D812E, appears to be nonspecific. The K(d) values of the Asp → Ash mutants are only 2-5 times larger than the value for the wild-type, in contrast to the drastic diminution of enzymatic activity in the mutants. The geometry of metal binding to these Asp residues may be crucial in determining the catalytic competence. Mn2+ binding to the wild-type enzyme in the presence of nucleotides, measured by flow dialysis, is characterized by two Mn2+- binding sites with a K(d) value of ca. 150 μM. The similarity in values of K(d) with and without nucleotide suggests that nucleotides do not have a drastic effect on Mn2+ binding. Our results indicate that monodentate carboxylate oxygens of both conserved Asp residues bridge the two metal ions.

Original languageEnglish (US)
Pages (from-to)132-143
Number of pages12
Issue number1
StatePublished - Jan 9 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry


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