Effect of rhombinant endothelial nitric oxide synthase gene exprkssion on ri activity of isolated canine bash ar arthry

A. I.Y. Chen, H. Kinoshita, M. Tsutsui, T. O'Bnen, V. J. Pompili, I. B. Crottv, S. Katusic

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The present stud\ was designed to determine whether expression of recombinant endothelial nitric oxide synthase (eNOS) may affect vasomotor iunaion of canine cerebral arteries- Replication-defective adenoviral vectors encoding hovine eNOS (AdCMVNOS) gene and bacterial -galactosidasc (AdCMVLau/) gene were constructed. Kx vivo gene transfer was achieved by 30 mm incubation ofbasibr artery rings in minimal essential medium {37°C) increasing.! liters ) 10'. and 3.5 x 101" pfu/ml) of adenoviral vectors. Foru -eight hours following gene transier arteries were examined for lunuionai expression of recombinant gene, using histochemical staining, iMiinetric tension recordings and c\eiic GMP radioimmunoassay. Transfection u ith -\dC MVl.acX or AdCMVNOS resulted in a viral tiler-dependent increase MI reconibinant protein expression m adventitia. In arteries transfected with Ad< MVNOS (but not AdCMVl ac/) contractile responses to uridine 5'inph<>sphate 110 -]0 'Ml were significantly reduced. ['Expression of K.-c.mibtnant -Vdt'MX'NOS resulted in increased basal production of cyclic ( 'MP that vvu-, inhibited by Nl'-monnmethyl-l-arginine. These results suggest !!u': ai successful expression oi'recoinbinant AdCMVNOS can be achieved in iCiebral arteries, and b) eNOS gene transfer may provide a novel approach to icsuire impaired niini- oxide production in diseased blood vessels.

Original languageEnglish (US)
Pages (from-to)A303
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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