Effects of chemokine MCP-1 on NMDA-mediated exciatory postsynaptic current in hippocampal slice of rats

Shan Li, Zhe Hu, Yan Zhou, Huan Gui Xiong

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Aim To explore how MCP-1 induces neurodisorder by determing the effects of MCP-1 on excitatory postsynaptic current ( EPSCs) in the CA1 region of rat hippocampal brain slices. Methods EPSCs, the AMPA receptor-mediated EPSC ( EPSCampar ), NMDA receptor mediated EPSCs(EPSCNMDAR) and NR2BR receptor-mediated EPSC ( EPSCNR2BR ) were recorded using whole-cell patch recording techniques to observe the effects of 2. 3 nmol • L-1 MCP-1 on pyramidal neurons in hippocampal CA1 region. Microtubule-Associated protein-2 ( MAP-2 ) staining was used to study whether MCP-1 induced dendritic injuries in hippocampal CA1 region and whether NMDAR, AMPAR or CCR2 receptor antagonists had protective effects against dendritic damage caused by MCP-1. Results (J) Bath application of MCP-1 produced a significant enhancement of the amplitudes of EPSCs, EPSCampar and EPSCnmdar. (2) Further studies revealed that MCP- 1 potentiated EPSCNR2BR; (3) The MCP-1-Associated dendritic injuries were blocked by NMDAR, AMPAR and CCR2R antagonists respectively. Conclusions Our results suggest a potential role of MCP-1 which may play in neuroexcitotoxicity and neural injury via NMDA receptor (especially NMDAR subtype NR2BR) and CCR2 receptor. The antagonists of these receptors may have potential therapeutic effect for neurodegeneration.

Original languageEnglish (US)
Pages (from-to)950-955
Number of pages6
JournalChinese Pharmacological Bulletin
Volume32
Issue number7
DOIs
StatePublished - Jul 2016

Keywords

  • Chemokine
  • EPSCs
  • HAND
  • Hippocampal slices
  • MCP-1
  • Neuroexcitoxicity

ASJC Scopus subject areas

  • Pharmacology

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