Effects of deep hypothermic circulatory arrest on the blood brain barrier in a cardiopulmonary bypass model - a pilot study

Karsten Bartels, Qing Ma, Talaignair N. Venkatraman, Christopher R. Campos, Lindsay Smith, Ronald E. Cannon, Mihai V. Podgoreanu, Christopher D. Lascola, David S. Miller, Joseph P. Mathew

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Background: Neurologic injury is common after cardiac surgery and disruption of the blood brain barrier (BBB) has been proposed as a contributing factor. We sought to study BBB characteristics in a rodent model of cardiopulmonary bypass (CPB) and deep hypothermic circulatory arrest (DHCA). Methods: Adult rats were subjected to CPB/DHCA or to sham surgery. Analysis included Western blotting of relevant BBB proteins in addition to in vivo brain magnetic resonance imaging (MRI) with a clinically used low-molecular contrast agent. Results: While quantitative analysis of BBB proteins revealed similar expression levels, MRI showed evidence of BBB disruption after CPB/DHCA compared to sham surgery. Conclusions: Combining molecular BBB analysis and MRI technology in a rodent model is a highly translatable approach to study adverse neurologic outcomes following CPB/DHCA.

Original languageEnglish (US)
Pages (from-to)981-984
Number of pages4
JournalHeart Lung and Circulation
Volume23
Issue number10
DOIs
StatePublished - Oct 1 2014
Externally publishedYes

Keywords

  • Blood brain barrier
  • Cardiac surgery
  • Cardiopulmonary bypass
  • Deep hypothermic circulatory arrest
  • Neurologic injury

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

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