Abstract
Background: Neurologic injury is common after cardiac surgery and disruption of the blood brain barrier (BBB) has been proposed as a contributing factor. We sought to study BBB characteristics in a rodent model of cardiopulmonary bypass (CPB) and deep hypothermic circulatory arrest (DHCA). Methods: Adult rats were subjected to CPB/DHCA or to sham surgery. Analysis included Western blotting of relevant BBB proteins in addition to in vivo brain magnetic resonance imaging (MRI) with a clinically used low-molecular contrast agent. Results: While quantitative analysis of BBB proteins revealed similar expression levels, MRI showed evidence of BBB disruption after CPB/DHCA compared to sham surgery. Conclusions: Combining molecular BBB analysis and MRI technology in a rodent model is a highly translatable approach to study adverse neurologic outcomes following CPB/DHCA.
Original language | English (US) |
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Pages (from-to) | 981-984 |
Number of pages | 4 |
Journal | Heart Lung and Circulation |
Volume | 23 |
Issue number | 10 |
DOIs | |
State | Published - Oct 1 2014 |
Externally published | Yes |
Keywords
- Blood brain barrier
- Cardiac surgery
- Cardiopulmonary bypass
- Deep hypothermic circulatory arrest
- Neurologic injury
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Cardiology and Cardiovascular Medicine