Effects of in vitro ethanol on the brain cation pump in alcoholics and controls

John M. Bertoni, Pamela M. Sprenkle

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

In vivo ethanol exposure reduces in vitro Na+K+-adenosine triphosphatase (Na+K+-ATPase) sensitivity to ethanol in some animal models, but very little is known about the effects of ethanol on human brain Na+K+-ATPase. Cerebral cortex homogenates from 13 male alcoholic and 9 control subjects were assayed for K+-p-nitrophenylphosphatase (K+-pNPPase, a measure of Na+K+ ATPase) and Mg2+-pNPPase activities at 37° for 20 min in 75 mM imidazole-HCl (pH7.4), 5mM p-nitrophenylphosphate, 5mM MgCl2, and 20 mM KCl, with or without 1 mM ouabain. Native K+-pNPPase activities were similar in control and alcoholic brains (61.5 ± 3.5 vs 55.3 ± 3.1 nmol/mg/min). In vitro exposure to a near lethal ethanol level (0.5%, or 110 mM) was without effect, whereas 5% ethanol inhibited IC-pNPPase activity by about 28% (P < 0.001) in both groups. Both 0.5 and 5% ethanol in vitro significantly stimulated Mg2+-pNPPase activity (1-2% and 19-20%, respectively). By comparison, mouse brain K+-pNPPase was inhibited significantly by in vitro ethanol, and Mg2+-pNPPase activity was unaffected. Ethanol levels attainable in humans may not be sufficient to alter significantly brain Na+,K+-ATPase activity.

Original languageEnglish (US)
Pages (from-to)435-437
Number of pages3
JournalBiochemical Pharmacology
Volume48
Issue number2
DOIs
StatePublished - Jul 19 1994

Keywords

  • K-activated p-nitro-phenylphosphatase
  • K-adenosine triphosphatase
  • Na
  • alcohol
  • brain
  • cation pump
  • tolerance

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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