TY - JOUR
T1 - Effects of metals Cu, Fe, Ni, V, and Zn on rat lung epithelial cells
AU - Riley, Mark R.
AU - Boesewetter, Dianne E.
AU - Kim, Aana M.
AU - Sirvent, Francisco P.
N1 - Funding Information:
This work was supported by an NIEHS sponsored Southwest Environmental Health Sciences Center Pilot Project #P30 ES06694 and by DARPA contract #N66001-C-8041.
PY - 2003/8/28
Y1 - 2003/8/28
N2 - Inhalation of combustion-derived particulate matter can have a variety of negative impacts on human health. Metals are known to play a substantial role in these effects, however, the interactions between cellular responses caused by multiple metals is not well understood. The impact of metals (Zn, Cu, Ni, V, and Fe) individually and in combination on a rat lung epithelial cell line (RLE-6TN) was evaluated. Quantifications involved measurement of inhibition of cell culture metabolism (mitochondrial succinate dehydrogenase activity), cell death, mechanisms of cell death, and cytokine secretion. The ranking of metal toxicity based on TC50 values is V>Zn>Cu>Ni>Fe. Interactions were observed for exposures containing multiple metals: Zn+V, Zn+Cu, Zn+Fe, and Zn+Ni. Zn appears to diminish the negative impact of V and Cu; has an additive effect with Ni, and has no substantial effect on Fe toxicity.
AB - Inhalation of combustion-derived particulate matter can have a variety of negative impacts on human health. Metals are known to play a substantial role in these effects, however, the interactions between cellular responses caused by multiple metals is not well understood. The impact of metals (Zn, Cu, Ni, V, and Fe) individually and in combination on a rat lung epithelial cell line (RLE-6TN) was evaluated. Quantifications involved measurement of inhibition of cell culture metabolism (mitochondrial succinate dehydrogenase activity), cell death, mechanisms of cell death, and cytokine secretion. The ranking of metal toxicity based on TC50 values is V>Zn>Cu>Ni>Fe. Interactions were observed for exposures containing multiple metals: Zn+V, Zn+Cu, Zn+Fe, and Zn+Ni. Zn appears to diminish the negative impact of V and Cu; has an additive effect with Ni, and has no substantial effect on Fe toxicity.
KW - Cell death
KW - Epithelial cell
KW - Particulate matter
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U2 - 10.1016/S0300-483X(03)00162-8
DO - 10.1016/S0300-483X(03)00162-8
M3 - Article
C2 - 12927373
AN - SCOPUS:0042133199
SN - 0300-483X
VL - 190
SP - 171
EP - 184
JO - Toxicology
JF - Toxicology
IS - 3
ER -