Effects of mustine hydrochloride on mouse haematopoietic and lymphoid tissues

During the first 4 days after the intravenous administration of a lethal dose (100 μg) of mustine hydrochloride to mice, there is a marked decrease in the weights of the lymph nodes, thymus and spleen and in the cellularity of the femoral bone marrow. In the subsequent 4 days, the cellularity of the bone marrow increases to values within the normal range while the weights of the lymph nodes, thymus and spleen remain exceedingly small. In this bone marrow regenerating after the injection of mustine hydrochloride, 94% of the recognisable haematopoietic cells are granulocytes at various stages of maturation.