Effects of oxidation and reduction on the binding of transcription factors to cis-regulatory elements located in the FGF-4 gene

Kim Lickteig, Kimberly Lamb, Kristen Brigman, Angie Rizzino

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Previous studies have shown that the addition of reducing agents to the culture medium of embryonic cell lines stimulates their growth. Moreover, recent studies have shown that the redox state of several transcription factors affects their binding to DNA. In light of these findings, we employed gel mobility shift analysis to examine the effects of oxidation and reduction on the ability of transcription factors to bind cis-regulatory elements located in the FGF-4 gene, which is expressed during early mammalian development. In this study, we demonstrate that both the oxidizing agent diamide and the alkylating agent N-ethylmaleimide inhibit the ability of Oct- 1, Oct-3, Sp1, and several Sp1-related nuclear proteins to bind important cis-regulatory elements located in the FGF-4 gene. We also demonstrate that not all transcription factors are affected by oxidation. Specifically, we show that the binding of the transcription factor NF-YA, which binds to a critical CCAAT box, and the binding of a high mobility group (HMG) protein(s), which binds to a critical HMG motif, are not affected by diamide or N-ethylmaleimide. Taken together, our findings and those of others support the hypothesis that the redox state of the cell can regulate gene transcription and, thus, can influence important physiological processes.

Original languageEnglish (US)
Pages (from-to)146-152
Number of pages7
JournalMolecular Reproduction and Development
Volume44
Issue number2
DOIs
StatePublished - Jun 1996

Keywords

  • DNA binding
  • Embryonal carcinoma cells
  • Gene expression
  • Octamer binding proteins
  • Redox

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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