TY - JOUR
T1 - Effects of redox label location on the performance of an electrochemical aptamer-based tumor necrosis factor-alpha sensor
AU - Mayer, Miles D.
AU - Lai, Rebecca Y.
N1 - Funding Information:
This work was supported by the National Science Foundation ( CHE-0955439 ) and Nebraska EPSCoR ( EPS-1004094 ). The authors would like to thank Dr. Anita J. Zaitouna, Channing C. Thompson, and Dr. Yao Wu for the helpful discussions. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
PY - 2018/11/1
Y1 - 2018/11/1
N2 - We report the development of an electrochemical aptamer-based sensor for real time detection of tumor necrosis factor-alpha. The focus of this study is to evaluate the effects of the redox label location on the overall sensor performance, including sensor stability, detection limit, reusability, and selectivity. Three aptamer probes, each labeled with methylene blue (MB) at a specific location, were designed and employed in the fabrication of the sensors. Among the three sensors, the sensor fabricated using an aptamer with the MB label located at the distal end has a detection limit of 100 pM and is regenerable. The sensor fabricated using an aptamer with an internal MB modification has a detection limit of 10 nM and is not regenerable. Both sensors can be employed in complex biological samples such as 50% urine and 50% saliva. However, the sensor fabricated with an aptamer with the MB label located at the proximal end suffers from poor reproducibility and is highly unstable, thus limiting its application as a sensor. On the bases of these results, placing the MB label at the distal end of the aptamer probe appears to be the most advantageous for this sensor design for it does not interfere with monolayer formation and target binding.
AB - We report the development of an electrochemical aptamer-based sensor for real time detection of tumor necrosis factor-alpha. The focus of this study is to evaluate the effects of the redox label location on the overall sensor performance, including sensor stability, detection limit, reusability, and selectivity. Three aptamer probes, each labeled with methylene blue (MB) at a specific location, were designed and employed in the fabrication of the sensors. Among the three sensors, the sensor fabricated using an aptamer with the MB label located at the distal end has a detection limit of 100 pM and is regenerable. The sensor fabricated using an aptamer with an internal MB modification has a detection limit of 10 nM and is not regenerable. Both sensors can be employed in complex biological samples such as 50% urine and 50% saliva. However, the sensor fabricated with an aptamer with the MB label located at the proximal end suffers from poor reproducibility and is highly unstable, thus limiting its application as a sensor. On the bases of these results, placing the MB label at the distal end of the aptamer probe appears to be the most advantageous for this sensor design for it does not interfere with monolayer formation and target binding.
KW - Alternating current voltammetry
KW - Electrochemical aptamer-based sensor
KW - Methylene blue
KW - Redox label location
KW - Self-assembled monolayer
KW - Tumor necrosis factor-alpha
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U2 - 10.1016/j.talanta.2018.07.055
DO - 10.1016/j.talanta.2018.07.055
M3 - Article
C2 - 30086964
AN - SCOPUS:85050210529
VL - 189
SP - 585
EP - 591
JO - Talanta
JF - Talanta
SN - 0039-9140
ER -