Efficacy of multiple administrations of a recombinant adenovirus expressing wild-type p53 in an immune-competent mouse tumor model

Z. Li, A. Rakkar, Y. Katayose, M. Kim, N. Shanmugam, S. Srivastava, J. W. Moul, D. G. McLeod, K. H. Cowan, P. Seth

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Infection of Renca cells in vitro with a recombinant adenovirus expressing a marker gene β-galactosidase resulted in high level of the transgene expression. Renca tumors grown in Balb/C mice were also infectable with this recombinant adenovirus. The transgene expression in the tumors lasted for about 7 days, however, administration of another of Ad-βgal, on day 7 produced β-galactosidase expression. To investigate the effect of antibodies to adenovirus, animals were injected with multiple doses of adenovirus to produce neutralizing antibodies. To these animals Renca cells were injected and tumors formed. Interestingly, when Adβ-gal was administered into these tumors, a high level of transgene expression was still observed. We next explored the utility of a recombinant adenovirus expressing p53 (AdWTp53) in the Renca tumor model. Renca cells when exposed to an adenovirus expressing p53 (AdWTp53) produced a high level of p53 protein, a p53-inducible gene p21/WAF1/Cip1 and underwent apoptosis. A single injection of AdWTp53 (109 plaque forming units) resulted in significant inhibition of tumor growth. However, multiple administrations (four doses of 2.5 x 108 plaque forming units) of AdWTp53 were needed for tumor cures. Mixing uninfected and AdWTp53-infected cells showed a bystander effect of AdWTp53-infected Renca cells. Based on these results we believe that an appropriate dose scheduling of AdWTp53 can be efficacious for cancer gene therapy in immune-competent tumor-bearing animals.

Original languageEnglish (US)
Pages (from-to)605-613
Number of pages9
JournalGene Therapy
Volume5
Issue number5
DOIs
StatePublished - Jan 1 1998

Keywords

  • Adenovirus
  • Apoptosis
  • Bystander effect
  • Gene therapy
  • p53

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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    Li, Z., Rakkar, A., Katayose, Y., Kim, M., Shanmugam, N., Srivastava, S., Moul, J. W., McLeod, D. G., Cowan, K. H., & Seth, P. (1998). Efficacy of multiple administrations of a recombinant adenovirus expressing wild-type p53 in an immune-competent mouse tumor model. Gene Therapy, 5(5), 605-613. https://doi.org/10.1038/sj.gt.3300636