TY - JOUR
T1 - Efficiency of neutralizing antibodies targeting the CD4-binding site
T2 - Influence of conformational masking by the V2 loop in R5-tropic clade C simian-human immunodeficiency virus
AU - Watkins, Jennifer D.
AU - Diaz-Rodriguez, Juan
AU - Siddappa, Nagadenahalli B.
AU - Corti, Davide
AU - Ruprecht, Ruth M.
PY - 2011/12
Y1 - 2011/12
N2 - In R5-tropic clade C simian-human immunodeficiency viruses (SHIV-Cs), we identified a 3-asparagine (3N) deletion mutation in the V2 loop stem of gp120 as the major determinant of neutralization escape of the anti-CD4-binding site (anti-CD4-bs) neutralizing monoclonal antibody (nMAb) b12. However, the more potent anti-CD4-bs nMAbs VRC01 and VRC03 were not sensitive to this mutation. Using isogenic tier 1 or tier 2 proviruses differing only in the 3N mutation, we showed that this mutation might result in selective conformational b12 epitope masking. Therefore, human immunodeficiency virus (HIV) Env immunogens targeting the CD4-bs and designed to neutralize tier 2 viruses should take conformational masking by the V2 loop into account.
AB - In R5-tropic clade C simian-human immunodeficiency viruses (SHIV-Cs), we identified a 3-asparagine (3N) deletion mutation in the V2 loop stem of gp120 as the major determinant of neutralization escape of the anti-CD4-binding site (anti-CD4-bs) neutralizing monoclonal antibody (nMAb) b12. However, the more potent anti-CD4-bs nMAbs VRC01 and VRC03 were not sensitive to this mutation. Using isogenic tier 1 or tier 2 proviruses differing only in the 3N mutation, we showed that this mutation might result in selective conformational b12 epitope masking. Therefore, human immunodeficiency virus (HIV) Env immunogens targeting the CD4-bs and designed to neutralize tier 2 viruses should take conformational masking by the V2 loop into account.
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U2 - 10.1128/JVI.05994-11
DO - 10.1128/JVI.05994-11
M3 - Comment/debate
C2 - 21957314
AN - SCOPUS:81255167375
SN - 0022-538X
VL - 85
SP - 12811
EP - 12814
JO - Journal of virology
JF - Journal of virology
IS - 23
ER -