Electrochemical hydrogen peroxide sensors fabricated using cytochrome c immobilized on macroelectrodes and ultramicroelectrodes

S. Ehsan Salamifar, Stephen Lee, Rebecca Y. Lai

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We report the design and fabrication of hydrogen peroxide (H2O2) sensors using heme proteins immobilized on macroelectrodes and ultramicroelectrodes (UMEs). In this sensor design, the heme centers are directly "wired" to the electrode via the use of an imidazole-terminated self-assembled monolayer. We have systematically evaluated the effect of electrode type and size on sensor performance. The limit of detection for H2O2 determined using a 10-μm gold UME is significantly lower than that obtained using a stationary macroelectrode. Our results also highlight the advantages of using UMEs for enzyme kinetics analysis; the Km determined using a 10-μm UME is similar to that obtained from a rotating disk electrode.

Original languageEnglish (US)
Pages (from-to)866-869
Number of pages4
JournalColloids and Surfaces B: Biointerfaces
Volume123
DOIs
StatePublished - Nov 1 2014

Keywords

  • Cytochrome c
  • Electron transfer rate
  • Hydrogen peroxide
  • Michaelis-Menten kinetics
  • Rotating disk electrodes
  • Ultramicroelectrodes

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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