Electrochemical hydrogen peroxide sensors fabricated using cytochrome c immobilized on macroelectrodes and ultramicroelectrodes

S. Ehsan Salamifar; Stephen Lee; Rebecca Y. Lai

doi:10.1016/j.colsurfb.2014.10.033

Electrochemical hydrogen peroxide sensors fabricated using cytochrome c immobilized on macroelectrodes and ultramicroelectrodes

S. Ehsan Salamifar, Stephen Lee, Rebecca Y. Lai

Chemistry

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

We report the design and fabrication of hydrogen peroxide (H₂O₂) sensors using heme proteins immobilized on macroelectrodes and ultramicroelectrodes (UMEs). In this sensor design, the heme centers are directly "wired" to the electrode via the use of an imidazole-terminated self-assembled monolayer. We have systematically evaluated the effect of electrode type and size on sensor performance. The limit of detection for H₂O₂ determined using a 10-μm gold UME is significantly lower than that obtained using a stationary macroelectrode. Our results also highlight the advantages of using UMEs for enzyme kinetics analysis; the K_m determined using a 10-μm UME is similar to that obtained from a rotating disk electrode.

Original language	English (US)
Pages (from-to)	866-869
Number of pages	4
Journal	Colloids and Surfaces B: Biointerfaces
Volume	123
DOIs	https://doi.org/10.1016/j.colsurfb.2014.10.033
State	Published - Nov 1 2014

Keywords

Cytochrome c
Electron transfer rate
Hydrogen peroxide
Michaelis-Menten kinetics
Rotating disk electrodes
Ultramicroelectrodes

ASJC Scopus subject areas

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2014.10.033

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title = "Electrochemical hydrogen peroxide sensors fabricated using cytochrome c immobilized on macroelectrodes and ultramicroelectrodes",

abstract = "We report the design and fabrication of hydrogen peroxide (H2O2) sensors using heme proteins immobilized on macroelectrodes and ultramicroelectrodes (UMEs). In this sensor design, the heme centers are directly {"}wired{"} to the electrode via the use of an imidazole-terminated self-assembled monolayer. We have systematically evaluated the effect of electrode type and size on sensor performance. The limit of detection for H2O2 determined using a 10-μm gold UME is significantly lower than that obtained using a stationary macroelectrode. Our results also highlight the advantages of using UMEs for enzyme kinetics analysis; the Km determined using a 10-μm UME is similar to that obtained from a rotating disk electrode.",

keywords = "Cytochrome c, Electron transfer rate, Hydrogen peroxide, Michaelis-Menten kinetics, Rotating disk electrodes, Ultramicroelectrodes",

author = "Salamifar, {S. Ehsan} and Stephen Lee and Lai, {Rebecca Y.}",

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doi = "10.1016/j.colsurfb.2014.10.033",

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T1 - Electrochemical hydrogen peroxide sensors fabricated using cytochrome c immobilized on macroelectrodes and ultramicroelectrodes

AU - Salamifar, S. Ehsan

AU - Lee, Stephen

AU - Lai, Rebecca Y.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - We report the design and fabrication of hydrogen peroxide (H2O2) sensors using heme proteins immobilized on macroelectrodes and ultramicroelectrodes (UMEs). In this sensor design, the heme centers are directly "wired" to the electrode via the use of an imidazole-terminated self-assembled monolayer. We have systematically evaluated the effect of electrode type and size on sensor performance. The limit of detection for H2O2 determined using a 10-μm gold UME is significantly lower than that obtained using a stationary macroelectrode. Our results also highlight the advantages of using UMEs for enzyme kinetics analysis; the Km determined using a 10-μm UME is similar to that obtained from a rotating disk electrode.

AB - We report the design and fabrication of hydrogen peroxide (H2O2) sensors using heme proteins immobilized on macroelectrodes and ultramicroelectrodes (UMEs). In this sensor design, the heme centers are directly "wired" to the electrode via the use of an imidazole-terminated self-assembled monolayer. We have systematically evaluated the effect of electrode type and size on sensor performance. The limit of detection for H2O2 determined using a 10-μm gold UME is significantly lower than that obtained using a stationary macroelectrode. Our results also highlight the advantages of using UMEs for enzyme kinetics analysis; the Km determined using a 10-μm UME is similar to that obtained from a rotating disk electrode.

KW - Cytochrome c

KW - Electron transfer rate

KW - Hydrogen peroxide

KW - Michaelis-Menten kinetics

KW - Rotating disk electrodes

KW - Ultramicroelectrodes

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DO - 10.1016/j.colsurfb.2014.10.033

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SN - 0927-7765

VL - 123

SP - 866

EP - 869

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

Electrochemical hydrogen peroxide sensors fabricated using cytochrome c immobilized on macroelectrodes and ultramicroelectrodes

Abstract

Keywords

ASJC Scopus subject areas

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