TY - JOUR
T1 - Electrospun thymosin Beta-4 loaded PLGA/PLA nanofiber/ microfiber hybrid yarns for tendon tissue engineering application
AU - Wu, Shaohua
AU - Zhou, Rong
AU - Zhou, Fang
AU - Streubel, Philipp N.
AU - Chen, Shaojuan
AU - Duan, Bin
N1 - Funding Information:
This work has been supported by Mary & Dick Holland Regenerative Medicine Program start-up grant, Nebraska Research Initiative funding , and National Institutes of Health ( R01 AR073225 ) to B.D.. S.C. thanks for the support from Chinese science and technology major projects ( 2017YFB0309805-02 and 2018GGX108003 ). Support for the UNMC Advanced Microscopy Core Facility was provided by the Nebraska Research Initiative , the Fred and Pamela Buffett Cancer Center Support Grant ( P30CA036727 ), and an Institutional Development Award (IDeA) from the NIGMS of the NIH ( P30GM106397 ). The authors declare no competing financial interest.
Funding Information:
This work has been supported by Mary & Dick Holland Regenerative Medicine Program start-up grant, Nebraska Research Initiative funding, and National Institutes of Health (R01 AR073225) to B.D. S.C. thanks for the support from Chinese science and technology major projects (2017YFB0309805-02 and 2018GGX108003). Support for the UNMC Advanced Microscopy Core Facility was provided by the Nebraska Research Initiative, the Fred and Pamela Buffett Cancer Center Support Grant (P30CA036727), and an Institutional Development Award (IDeA) from the NIGMS of the NIH (P30GM106397). The authors declare no competing financial interest.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/1
Y1 - 2020/1
N2 - Microfiber yarns (MY) have been widely employed to construct tendon tissue grafts. However, suboptimal ultrastructure and inappropriate environments for cell interactions limit their clinical application. Herein, we designed a modified electrospinning device to coat poly(lactic-co-glycolic acid) PLGA nanofibers onto polylactic acid (PLA) MY to generate PLGA/PLA hybrid yarns (HY), which had a well-aligned nanofibrous structure, resembling the ultrastructure of native tendon tissues and showed enhanced failure load compared to PLA MY. PLGA/PLA HY significantly improved the growth, proliferation, and tendon-specific gene expressions of human adipose derived mesenchymal stem cells (HADMSC) compared to PLA MY. Moreover, thymosin beta-4 (Tβ4) loaded PLGA/PLA HY presented a sustained drug release manner for 28 days and showed an additive effect on promoting HADMSC migration, proliferation, and tenogenic differentiation. Collectively, the combination of Tβ4 with the nano-topography of PLGA/PLA HY might be an efficient strategy to promote tenogenesis of adult stem cells for tendon tissue engineering.
AB - Microfiber yarns (MY) have been widely employed to construct tendon tissue grafts. However, suboptimal ultrastructure and inappropriate environments for cell interactions limit their clinical application. Herein, we designed a modified electrospinning device to coat poly(lactic-co-glycolic acid) PLGA nanofibers onto polylactic acid (PLA) MY to generate PLGA/PLA hybrid yarns (HY), which had a well-aligned nanofibrous structure, resembling the ultrastructure of native tendon tissues and showed enhanced failure load compared to PLA MY. PLGA/PLA HY significantly improved the growth, proliferation, and tendon-specific gene expressions of human adipose derived mesenchymal stem cells (HADMSC) compared to PLA MY. Moreover, thymosin beta-4 (Tβ4) loaded PLGA/PLA HY presented a sustained drug release manner for 28 days and showed an additive effect on promoting HADMSC migration, proliferation, and tenogenic differentiation. Collectively, the combination of Tβ4 with the nano-topography of PLGA/PLA HY might be an efficient strategy to promote tenogenesis of adult stem cells for tendon tissue engineering.
KW - Cell migration
KW - Core-sheath yarn
KW - Drug delivery
KW - Nanofiber yarn
KW - Tenogenic differentiation
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U2 - 10.1016/j.msec.2019.110268
DO - 10.1016/j.msec.2019.110268
M3 - Article
C2 - 31753373
AN - SCOPUS:85073216814
SN - 0928-4931
VL - 106
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
M1 - 110268
ER -