Endoplasmic reticulum stress pathway mediates the early heat stress response of developing rice seeds

Jaspreet Sandhu, Larissa Irvin, Kan Liu, Paul Staswick, Chi Zhang, Harkamal Walia

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A transient heat stress occurring during early seed development in rice (Oryza sativa) reduces seed size by altering endosperm development. However, the relationship between the timing of the stress and specific developmental stage on heat sensitivity is not well-understood. To address this, we imposed a series of non-overlapping heat stress treatments and found that young seeds are most sensitive during the first two days after flowering. Temporal transcriptome analysis of developing, heat stressed (35°C) seeds during this window shows that Inositol-requiring enzyme 1 (IRE1)-mediated endoplasmic reticulum (ER) stress response and jasmonic acid (JA) pathways are the early (1–3 h) drivers of heat stress response. We propose that increased JA levels under heat stress may precede ER stress response as JA application promotes the spliced form of OsbZIP50, an ER response marker gene linked to IRE1-specific pathway. This study presents temporal and mechanistic insights into the role of JA and ER stress signalling during early heat stress response of rice seeds that impact both grain size and quality. Modulating the heat sensitivity of the early sensing pathways and downstream endosperm development genes can enhance rice resilience to transient heat stress events.

Original languageEnglish (US)
Pages (from-to)2604-2624
Number of pages21
JournalPlant Cell and Environment
Volume44
Issue number8
DOIs
StatePublished - Aug 2021

Keywords

  • cell cycle
  • coenocytic endosperm
  • jasmonic acid
  • seed development
  • temporal-transcriptome

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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