Energetic and hydration contributions of the removal of methyl groups from thymine to form uracil in G-quadruplexes

Chris M. Olsen; Luis A. Marky

doi:10.1021/jp808526d

Energetic and hydration contributions of the removal of methyl groups from thymine to form uracil in G-quadruplexes

Chris M. Olsen, Luis A. Marky

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

A combination of spectroscopic and calorimetric techniques is used to investigate the unfolding of two G-quadruplexes: d(G₂U ₂G₂UGUG₂U₂G₂), G2-U, and d(G₂T₂G₂TGTG₂T₂G ₂), G2. The comparisons of their thermodynamic data allow us to elucidate the role of methylation on the energetic and hydration properties accompanying their stable formation. The favorable formation of each G-quadruplex results from the characteristic enthalpy-entropy compensation, uptake of ions, and release of water molecules. The loops of G2-U and G2 contribute favorably to their formation, and the absence of methyl groups stabilizes the G-quadruplex. The unfolding of G2-U produces a larger ΔV, indicating a difference in the hydration states of the two oligonucleotides, while the opposite signs between ΔΔG° with the ΔΔV suggest that the differential hydration reflects structural, or hydrophobic, water is involved in the unfolding of G-quadruplexes.

Original language	English (US)
Pages (from-to)	9-11
Number of pages	3
Journal	Journal of Physical Chemistry B
Volume	113
Issue number	1
DOIs	https://doi.org/10.1021/jp808526d
State	Published - Jan 8 2009

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Surfaces, Coatings and Films
Materials Chemistry

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title = "Energetic and hydration contributions of the removal of methyl groups from thymine to form uracil in G-quadruplexes",

abstract = "A combination of spectroscopic and calorimetric techniques is used to investigate the unfolding of two G-quadruplexes: d(G2U 2G2UGUG2U2G2), G2-U, and d(G2T2G2TGTG2T2G 2), G2. The comparisons of their thermodynamic data allow us to elucidate the role of methylation on the energetic and hydration properties accompanying their stable formation. The favorable formation of each G-quadruplex results from the characteristic enthalpy-entropy compensation, uptake of ions, and release of water molecules. The loops of G2-U and G2 contribute favorably to their formation, and the absence of methyl groups stabilizes the G-quadruplex. The unfolding of G2-U produces a larger ΔV, indicating a difference in the hydration states of the two oligonucleotides, while the opposite signs between ΔΔG° with the ΔΔV suggest that the differential hydration reflects structural, or hydrophobic, water is involved in the unfolding of G-quadruplexes.",

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AU - Marky, Luis A.

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N2 - A combination of spectroscopic and calorimetric techniques is used to investigate the unfolding of two G-quadruplexes: d(G2U 2G2UGUG2U2G2), G2-U, and d(G2T2G2TGTG2T2G 2), G2. The comparisons of their thermodynamic data allow us to elucidate the role of methylation on the energetic and hydration properties accompanying their stable formation. The favorable formation of each G-quadruplex results from the characteristic enthalpy-entropy compensation, uptake of ions, and release of water molecules. The loops of G2-U and G2 contribute favorably to their formation, and the absence of methyl groups stabilizes the G-quadruplex. The unfolding of G2-U produces a larger ΔV, indicating a difference in the hydration states of the two oligonucleotides, while the opposite signs between ΔΔG° with the ΔΔV suggest that the differential hydration reflects structural, or hydrophobic, water is involved in the unfolding of G-quadruplexes.

AB - A combination of spectroscopic and calorimetric techniques is used to investigate the unfolding of two G-quadruplexes: d(G2U 2G2UGUG2U2G2), G2-U, and d(G2T2G2TGTG2T2G 2), G2. The comparisons of their thermodynamic data allow us to elucidate the role of methylation on the energetic and hydration properties accompanying their stable formation. The favorable formation of each G-quadruplex results from the characteristic enthalpy-entropy compensation, uptake of ions, and release of water molecules. The loops of G2-U and G2 contribute favorably to their formation, and the absence of methyl groups stabilizes the G-quadruplex. The unfolding of G2-U produces a larger ΔV, indicating a difference in the hydration states of the two oligonucleotides, while the opposite signs between ΔΔG° with the ΔΔV suggest that the differential hydration reflects structural, or hydrophobic, water is involved in the unfolding of G-quadruplexes.

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Energetic and hydration contributions of the removal of methyl groups from thymine to form uracil in G-quadruplexes

Abstract

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