Engaging challenges in glycoproteomics: Recent advances in MS-based glycopeptide analysis

Venkata Kolli; Katherine N. Schumacher; Eric D. Dodds

doi:10.4155/bio.14.272

Engaging challenges in glycoproteomics: Recent advances in MS-based glycopeptide analysis

Venkata Kolli, Katherine N. Schumacher, Eric D. Dodds

Chemistry

Research output: Contribution to journal › Review article › peer-review

53 Scopus citations

Abstract

The proteomic analysis of glycosylation is uniquely challenging. The numerous and varied biological roles of protein-linked glycans have fueled a tremendous demand for technologies that enable rapid, in-depth structural examination of glycosylated proteins in complex biological systems. In turn, this demand has driven many innovations in wide ranging fields of bioanalytical science. This review will summarize key developments in glycoprotein separation and enrichment, glycoprotein proteolysis strategies, glycopeptide separation and enrichment, the role of mass measurement accuracy in glycopeptide detection, glycopeptide ion dissociation methods for MS/MS, and informatic tools for glycoproteomic analysis. In aggregate, this selection of topics serves to encapsulate the present status of MS-based analytical technologies for engaging the challenges of glycoproteomic analysis.

Original language	English (US)
Pages (from-to)	113-131
Number of pages	19
Journal	Bioanalysis
Volume	7
Issue number	1
DOIs	https://doi.org/10.4155/bio.14.272
State	Published - Jan 1 2015

ASJC Scopus subject areas

Analytical Chemistry
General Pharmacology, Toxicology and Pharmaceutics
Medical Laboratory Technology
Clinical Biochemistry

Access to Document

10.4155/bio.14.272

Cite this

@article{3016d76ddf904e3f941393acb75a0c97,

title = "Engaging challenges in glycoproteomics: Recent advances in MS-based glycopeptide analysis",

abstract = "The proteomic analysis of glycosylation is uniquely challenging. The numerous and varied biological roles of protein-linked glycans have fueled a tremendous demand for technologies that enable rapid, in-depth structural examination of glycosylated proteins in complex biological systems. In turn, this demand has driven many innovations in wide ranging fields of bioanalytical science. This review will summarize key developments in glycoprotein separation and enrichment, glycoprotein proteolysis strategies, glycopeptide separation and enrichment, the role of mass measurement accuracy in glycopeptide detection, glycopeptide ion dissociation methods for MS/MS, and informatic tools for glycoproteomic analysis. In aggregate, this selection of topics serves to encapsulate the present status of MS-based analytical technologies for engaging the challenges of glycoproteomic analysis.",

author = "Venkata Kolli and Schumacher, {Katherine N.} and Dodds, {Eric D.}",

note = "Publisher Copyright: {\textcopyright} 2015 Future Science Ltd.",

year = "2015",

month = jan,

day = "1",

doi = "10.4155/bio.14.272",

language = "English (US)",

volume = "7",

pages = "113--131",

journal = "Bioanalysis",

issn = "1757-6180",

publisher = "Taylor and Francis Ltd.",

number = "1",

}

TY - JOUR

T1 - Engaging challenges in glycoproteomics

T2 - Recent advances in MS-based glycopeptide analysis

AU - Kolli, Venkata

AU - Schumacher, Katherine N.

AU - Dodds, Eric D.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - The proteomic analysis of glycosylation is uniquely challenging. The numerous and varied biological roles of protein-linked glycans have fueled a tremendous demand for technologies that enable rapid, in-depth structural examination of glycosylated proteins in complex biological systems. In turn, this demand has driven many innovations in wide ranging fields of bioanalytical science. This review will summarize key developments in glycoprotein separation and enrichment, glycoprotein proteolysis strategies, glycopeptide separation and enrichment, the role of mass measurement accuracy in glycopeptide detection, glycopeptide ion dissociation methods for MS/MS, and informatic tools for glycoproteomic analysis. In aggregate, this selection of topics serves to encapsulate the present status of MS-based analytical technologies for engaging the challenges of glycoproteomic analysis.

AB - The proteomic analysis of glycosylation is uniquely challenging. The numerous and varied biological roles of protein-linked glycans have fueled a tremendous demand for technologies that enable rapid, in-depth structural examination of glycosylated proteins in complex biological systems. In turn, this demand has driven many innovations in wide ranging fields of bioanalytical science. This review will summarize key developments in glycoprotein separation and enrichment, glycoprotein proteolysis strategies, glycopeptide separation and enrichment, the role of mass measurement accuracy in glycopeptide detection, glycopeptide ion dissociation methods for MS/MS, and informatic tools for glycoproteomic analysis. In aggregate, this selection of topics serves to encapsulate the present status of MS-based analytical technologies for engaging the challenges of glycoproteomic analysis.

UR - http://www.scopus.com/inward/record.url?scp=84920713443&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84920713443&partnerID=8YFLogxK

U2 - 10.4155/bio.14.272

DO - 10.4155/bio.14.272

M3 - Review article

C2 - 25558940

AN - SCOPUS:84920713443

SN - 1757-6180

VL - 7

SP - 113

EP - 131

JO - Bioanalysis

JF - Bioanalysis

IS - 1

ER -

Engaging challenges in glycoproteomics: Recent advances in MS-based glycopeptide analysis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this