TY - JOUR
T1 - Engineered Exosomes Containing Cathelicidin/LL-37 Exhibit Multiple Biological Functions
AU - Su, Yajuan
AU - Sharma, Navatha Shree
AU - John, Johnson V.
AU - Ganguli-Indra, Gitali
AU - Indra, Arup K.
AU - Gombart, Adrian F.
AU - Xie, Jingwei
N1 - Publisher Copyright:
© 2022 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.
PY - 2022/10/19
Y1 - 2022/10/19
N2 - Exosomes show great potential in diagnostic and therapeutic applications. Inspired by the human innate immune defense, herein, we report engineered exosomes derived from monocytic cells treated with immunomodulating compounds 1α,25-dihydroxyvitamin D3, and CYP24A1 inhibitor VID400 which are slowly released from electrospun nanofiber matrices. These engineered exosomes contain significantly more cathelicidin/LL-37 when compared with exosomes derived from either untreated cells or Cathelicidin Human Tagged ORF Clone transfected cells. In addition, such exosomes exhibit multiple biological functions evidenced by killing bacteria, facilitating human umbilical vein endothelial cell tube formation, and enhancing skin cell proliferation and migration. Taken together, the engineered exosomes developed in this study can be used as therapeutics alone or in combination with other biomaterials for effective infection management, wound healing, and tissue regeneration.
AB - Exosomes show great potential in diagnostic and therapeutic applications. Inspired by the human innate immune defense, herein, we report engineered exosomes derived from monocytic cells treated with immunomodulating compounds 1α,25-dihydroxyvitamin D3, and CYP24A1 inhibitor VID400 which are slowly released from electrospun nanofiber matrices. These engineered exosomes contain significantly more cathelicidin/LL-37 when compared with exosomes derived from either untreated cells or Cathelicidin Human Tagged ORF Clone transfected cells. In addition, such exosomes exhibit multiple biological functions evidenced by killing bacteria, facilitating human umbilical vein endothelial cell tube formation, and enhancing skin cell proliferation and migration. Taken together, the engineered exosomes developed in this study can be used as therapeutics alone or in combination with other biomaterials for effective infection management, wound healing, and tissue regeneration.
KW - antibacterial property
KW - engineered exosomes
KW - immunomodulating compounds
KW - nanofiber matrices
KW - wound healing
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U2 - 10.1002/adhm.202200849
DO - 10.1002/adhm.202200849
M3 - Article
C2 - 35930707
AN - SCOPUS:85135778651
SN - 2192-2640
VL - 11
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
IS - 20
M1 - 2200849
ER -