TY - GEN
T1 - Engineered nanostructured coatings for enhanced protein adsorption and cell growth
AU - Namavar, Fereydoon
AU - Rubinstein, Alexander
AU - Sabirianov, Renat F.
AU - Thiele, Geoffrey M.
AU - Sharp, J. Graham
AU - Pokharel, Utsav
AU - Namavar, Roxanna M.
AU - Garvin, Kevin L.
N1 - Funding Information:
We designed and produced pure cubic zirconia ceramic coatings by IBAD with nanostructures comparable to the size of proteins. Our ceramic coatings exhibit high hardness and superb hydrophilic properties, and, in contrast to HA, adhere well to all orthopaedic materials. Cell adhesion and proliferation experiments were performed with a bona fide mesenchymal stromal cell on the nano-structured coatings and compared to CoCr and HA. Our experimental results clearly indicated that nano-engineered cubic zirconia is superior in supporting adhesion and growth for mesenchymal stromal cells. First-principles quantum-mechanical calculations for the nanostructured ZrO2 have shown that, contrary to the smooth surface, the calculated charge density and the electrostatic potential vary significantly on the topological features of a nanostructured ZrO2 surface. The negatively charged surface features are readily interacting with the positively charged clusters located at the HBS of FN. The MC simulations clearly demonstrate higher adsorption energy of FN fragment for nano-engineered versus smooth ZrO2. ACKNOWLEDGMENTS We thank Nebraska Research Initiative, DoE, and NSF for the financial support.
PY - 2012
Y1 - 2012
N2 - We designed and produced pure cubic zirconia (ZrO 2) ceramic 1 coatings by an ion beam assisted deposition (IBAD) with nanostructures comparable to the size of proteins. Our ceramic coatings exhibit high hardness and a zero contact angle with serum. In contrast to hydroxyapatite (HA), nano-engineered zirconia films possess excellent adhesion to all orthopaedic materials. Cell adhesion and proliferation experiments were performed with a bona fide mesenchymal stromal cell line (OMA-AD). Our experimental results indicate that the nano-engineered cubic zirconia is superior in supporting growth, adhesion, and proliferation. Since cell attachment is mediated by adhesive proteins such as fibronectin (FN), to elucidate why cells attach more effectively to our nanostructures, we performed a comparative analysis of adsorption energies of FN fragment using quantum mechanical calculations and Monte Carlo (MC) simulation both on smooth and nanostructured surfaces. We have found that a FN fragment adsorbs significantly stronger on the nanostructured surface than on the smooth surface 2.
AB - We designed and produced pure cubic zirconia (ZrO 2) ceramic 1 coatings by an ion beam assisted deposition (IBAD) with nanostructures comparable to the size of proteins. Our ceramic coatings exhibit high hardness and a zero contact angle with serum. In contrast to hydroxyapatite (HA), nano-engineered zirconia films possess excellent adhesion to all orthopaedic materials. Cell adhesion and proliferation experiments were performed with a bona fide mesenchymal stromal cell line (OMA-AD). Our experimental results indicate that the nano-engineered cubic zirconia is superior in supporting growth, adhesion, and proliferation. Since cell attachment is mediated by adhesive proteins such as fibronectin (FN), to elucidate why cells attach more effectively to our nanostructures, we performed a comparative analysis of adsorption energies of FN fragment using quantum mechanical calculations and Monte Carlo (MC) simulation both on smooth and nanostructured surfaces. We have found that a FN fragment adsorbs significantly stronger on the nanostructured surface than on the smooth surface 2.
UR - http://www.scopus.com/inward/record.url?scp=84864968126&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864968126&partnerID=8YFLogxK
U2 - 10.1557/opl.2012.394
DO - 10.1557/opl.2012.394
M3 - Conference contribution
AN - SCOPUS:84864968126
SN - 9781605113951
T3 - Materials Research Society Symposium Proceedings
SP - 119
EP - 125
BT - Gels and Biomedical Materials
T2 - 2011 MRS Fall Meeting
Y2 - 28 November 2011 through 2 December 2011
ER -