Engineered nanostructured coatings for enhanced protein adsorption and cell growth

Fereydoon Namavar, Alexander Rubinstein, Renat F. Sabirianov, Geoffrey M. Thiele, J. Graham Sharp, Utsav Pokharel, Roxanna M. Namavar, Kevin L. Garvin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Scopus citations

Abstract

We designed and produced pure cubic zirconia (ZrO 2) ceramic 1 coatings by an ion beam assisted deposition (IBAD) with nanostructures comparable to the size of proteins. Our ceramic coatings exhibit high hardness and a zero contact angle with serum. In contrast to hydroxyapatite (HA), nano-engineered zirconia films possess excellent adhesion to all orthopaedic materials. Cell adhesion and proliferation experiments were performed with a bona fide mesenchymal stromal cell line (OMA-AD). Our experimental results indicate that the nano-engineered cubic zirconia is superior in supporting growth, adhesion, and proliferation. Since cell attachment is mediated by adhesive proteins such as fibronectin (FN), to elucidate why cells attach more effectively to our nanostructures, we performed a comparative analysis of adsorption energies of FN fragment using quantum mechanical calculations and Monte Carlo (MC) simulation both on smooth and nanostructured surfaces. We have found that a FN fragment adsorbs significantly stronger on the nanostructured surface than on the smooth surface 2.

Original languageEnglish (US)
Title of host publicationGels and Biomedical Materials
Pages119-125
Number of pages7
DOIs
StatePublished - 2012
Event2011 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 28 2011Dec 2 2011

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1418
ISSN (Print)0272-9172

Other

Other2011 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period11/28/1112/2/11

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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  • Cite this

    Namavar, F., Rubinstein, A., Sabirianov, R. F., Thiele, G. M., Sharp, J. G., Pokharel, U., Namavar, R. M., & Garvin, K. L. (2012). Engineered nanostructured coatings for enhanced protein adsorption and cell growth. In Gels and Biomedical Materials (pp. 119-125). (Materials Research Society Symposium Proceedings; Vol. 1418). https://doi.org/10.1557/opl.2012.394