TY - GEN
T1 - Engineering hydrogel based cancer microenvironments for in vitro breast cancer cell culture and therapeutics screening
AU - Shi, Wen
AU - Kuss, Mitchell A.
AU - Mohapatra, Bhopal
AU - Mirza, Sameer
AU - Band, Hamid
AU - Band, Vimla
AU - Duan, Bin
PY - 2019
Y1 - 2019
N2 - Statement of Purpose: Breast cancer cell of different invasiveness have been shown different morphologies and phenotypes when cultured inside various hydrogels1, 2. The commonly used 3D culture material-Matrigel suffers from issues such as batch to batch variation, complicated components and difficulty in stromal cell support. Type I collagen has offered an alternative to Matrigel for breast tumor culture, however, the morphology of breast cancer cells cultured in collagen is quite different from those in Matrigel. To solve such problem and better mimic the breast cancer microenvironment, we developed a novel collagen based hydrogel by mixing hyaluronic acid-poly (N-isopropylacrylamide) (HA-NIPAM) into collagen and evaluated the application of such hydrogel as a cancer microenvironment for in vitro breast cancer cell culture.
AB - Statement of Purpose: Breast cancer cell of different invasiveness have been shown different morphologies and phenotypes when cultured inside various hydrogels1, 2. The commonly used 3D culture material-Matrigel suffers from issues such as batch to batch variation, complicated components and difficulty in stromal cell support. Type I collagen has offered an alternative to Matrigel for breast tumor culture, however, the morphology of breast cancer cells cultured in collagen is quite different from those in Matrigel. To solve such problem and better mimic the breast cancer microenvironment, we developed a novel collagen based hydrogel by mixing hyaluronic acid-poly (N-isopropylacrylamide) (HA-NIPAM) into collagen and evaluated the application of such hydrogel as a cancer microenvironment for in vitro breast cancer cell culture.
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M3 - Conference contribution
AN - SCOPUS:85065397012
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
SP - 642
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
T2 - 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
Y2 - 3 April 2019 through 6 April 2019
ER -