Engineering of targeted nanoparticles for cancer therapy using internalizing aptamers isolated by cell-uptake selection

Zeyu Xiao, Etgar Levy-Nissenbaum, Frank Alexis, Andrej Lupták, Benjamin A. Teply, Juliana M. Chan, Jinjun Shi, Elise Digga, Judy Cheng, Robert Langer, Omid C. Farokhzad

Research output: Contribution to journalArticlepeer-review

128 Scopus citations

Abstract

Figure Persented: One of the major challenges in the development of targeted nanoparticles (NPs) for cancer therapy is to discover targeting ligands that allow for differential binding and uptake by the target cancer cells. Using prostate cancer (PCa) as a model disease, we developed a cell-uptake selection strategy to isolate PCa-specific internalizing 2′-O-methyl RNA aptamers (Apts) for NP incorporation. Twelve cycles of selection and counter-selection were done to obtain a panel of internalizing Apts, which can distinguish PCa cells from nonprostate and normal prostate cells. After Apt characterization, size minimization, and conjugation of the Apts with fluorescently labeled polymeric NPs, the NP-Apt conjugates exhibit PCa specificity and enhancement in cellular uptake when compared to nontargeted NPs lacking the internalizing Apts. Furthermore, when docetaxel, a chemotherapeutic agent used for the treatment of PCa, was encapsulated within the NP-Apt, a significant improvement in cytotoxicity was achieved in targeted PCa cells. Rather than isolating high-affinity Apts as reported in previous selection processes, our selection strategy was designed to enrich cancer cell-specific internalizing Apts. A similar cell-uptake selection strategy may be used to develop specific internalizing ligands for a myriad of other diseases and can potentially facilitate delivering various molecules, including drugs and siRNAs, into target cells.

Original languageEnglish (US)
Pages (from-to)696-704
Number of pages9
JournalACS Nano
Volume6
Issue number1
DOIs
StatePublished - Jan 24 2012

Keywords

  • aptamer
  • in vitro selection
  • internalization
  • nanoparticles
  • targeted cancer therapy

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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