Engineering thermoacidophilic archaea using linear DNA recombination

Yukari Maezato, Karl Dana, Paul Blum

Research output: Chapter in Book/Report/Conference proceedingChapter

17 Scopus citations

Abstract

Thermoacidophilic archaea comprise one of the major classes of extremophiles. Most belong to the family Sulfolobales within the phylum Crenarchaeota. They are of applied interest as sources of hyperstable enzymes, for biomining of base and precious metals, and for evolutionary studies because of their use of eukaryotic-like subcellular mechanisms. Genetic methods are available for several species particularly Sulfolobus solfataricus. This organism has a considerable number of methods available for the construction of novel cell lines with unique functions. This chapter presents recent developments in the use of homologous recombination and linear DNA for the engineering of site-specific changes in the genome of S. solfataricus.

Original languageEnglish (US)
Title of host publicationStrain Engineering
Subtitle of host publicationMethods and Protocols
EditorsJames Williams
Pages435-445
Number of pages11
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume765
ISSN (Print)1064-3745

Keywords

  • Archaeal recombineering
  • Cell line construction
  • Crenarchaeota
  • Hyperthermophiles
  • Linear DNA recombination

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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