Engraftment of MDR1 and NeoR gene-transduced hematopoietic cells after breast cancer chemotherapy

Jeffrey A. Moscow, Hui Huang, Charles Carter, Kenneth Hines, Jo Anne Zujewski, Georgie Cusack, Cathy Chow, David Venzon, Brian Sorrentino, Yawen Chiang, Barry Goldspiel, Susan Leitman, Elizabeth J. Read, Andrea Abati, Michael M. Gottesman, Ira Pastan, Stephanie Sellers, Cynthia Dunbar, Kenneth H. Cowan

Research output: Contribution to journalArticle

124 Scopus citations

Abstract

To determine whether the multidrug resistance gene MDR1 could act as a selectable marker in human subjects, we studied engraftment of peripheral blood progenitor cells (PBPCs) transduced with either MDR1 or the bacterial NeoR gene in six breast cancer patients. This study differed from previous MDR1 gene therapy studies in that patients received only PBPCs incubated in retroviral supernatants (no nonmanipulated PBPCs were infused), transduction of PBPCs was supported with autologous bone marrow stroma without additional cytokines, and a control gene (NeoR) was used for comparison with MDR1. Transduced PBPCs were infused after high-dose alkylating agent therapy and before chemotherapy with MDR-substrate drugs. We found that hematopoietic reconstitution can occur using only PBPCs incubated ex vivo, that the MDR1 gene product may play a role in engraftment, and that chemotherapy may selectively expand MDR1 gene-transduced hematopoietic cells relative to NeoR transduced cells in some patients.

Original languageEnglish (US)
Pages (from-to)52-61
Number of pages10
JournalBlood
Volume94
Issue number1
DOIs
StatePublished - Jul 1 1999

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Fingerprint Dive into the research topics of 'Engraftment of MDR1 and NeoR gene-transduced hematopoietic cells after breast cancer chemotherapy'. Together they form a unique fingerprint.

  • Cite this

    Moscow, J. A., Huang, H., Carter, C., Hines, K., Zujewski, J. A., Cusack, G., Chow, C., Venzon, D., Sorrentino, B., Chiang, Y., Goldspiel, B., Leitman, S., Read, E. J., Abati, A., Gottesman, M. M., Pastan, I., Sellers, S., Dunbar, C., & Cowan, K. H. (1999). Engraftment of MDR1 and NeoR gene-transduced hematopoietic cells after breast cancer chemotherapy. Blood, 94(1), 52-61. https://doi.org/10.1182/blood.v94.1.52.413k35_52_61