Enhanced antiproliferative activity by metastatic RAW117 lymphoma cells

S. S. Joshi, S. J. O'connor, D. D. Weisenburger, J. G. Sharp, H. M. Gharpure, K. W. Brunson

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The highly malignant/metastatic murine large cell lymphoma cell line RAW117-H10 forms 100-200 times more liver metastatic tumors than its parental counterpart cell line RAW117-P. RAW117-H10 cells, but not the less malignant/metastatic parental cells, significantly inhibited the mitogen-induced proliferation of normal syngeneic Balb/c and allogeneic ICRC mouse spleen cells. Such an inhibition also occurred when mitomycin-C treated metastatic lymphoma cells were added 24 h after initiation of culture, indicating that no competition with mitogen binding sites on the lymphocytes was necssary for inhibition of proliferation. 'Antiproliferative' cell surface molecules were extracted non-cytolytically from the RAW117-H10 cells using butanol. The butanol extracts from the metastatic RAW117-H10 cells also inhibited the mitogen-induced proliferation and natural killer (NK) cell-mediated cytotoxicity of normal spleen cells. Our results indicate that these 'antiproliferative' cell surface molecules of metastatic murine RAW117-H10 lymphoma cells may have important role(s) in tumor-mediated host immunosuppression.

Original languageEnglish (US)
Pages (from-to)27-37
Number of pages11
JournalClinical & Experimental Metastasis
Volume9
Issue number1
DOIs
StatePublished - Jan 1991

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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