Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts

M. G. Brattain; D. E. Brattain; A. M. Sarrif; L. J. McRae; W. D. Fine; J. G. Hawkins

Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts

M. G. Brattain, D. E. Brattain, A. M. Sarrif, L. J. McRae, W. D. Fine, J. G. Hawkins

Eppley Institute for Cancer Research

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The effects of feeder layers of C3H/10T 1/2 cells on the growth of human and mouse cell lines were tested. Feeder layers increased colony formation by cultured cancerous cells in semisolid medium over controls grown in semisolid medium without feeder layers. Maximal colony formation was also attained at a faster rate when feeder layers were used. Plating efficiency be cancerous cells obtained directly from xenografts was increased threefold to fivefold in tissue culture when feeder cells were present as confluent monolayers.

Original language	English (US)
Pages (from-to)	767-771
Number of pages	5
Journal	Journal of the National Cancer Institute
Volume	69
Issue number	4
State	Published - 1982

ASJC Scopus subject areas

Oncology
Cancer Research

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title = "Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts",

abstract = "The effects of feeder layers of C3H/10T 1/2 cells on the growth of human and mouse cell lines were tested. Feeder layers increased colony formation by cultured cancerous cells in semisolid medium over controls grown in semisolid medium without feeder layers. Maximal colony formation was also attained at a faster rate when feeder layers were used. Plating efficiency be cancerous cells obtained directly from xenografts was increased threefold to fivefold in tissue culture when feeder cells were present as confluent monolayers.",

author = "Brattain, {M. G.} and Brattain, {D. E.} and Sarrif, {A. M.} and McRae, {L. J.} and Fine, {W. D.} and Hawkins, {J. G.}",

year = "1982",

language = "English (US)",

volume = "69",

pages = "767--771",

journal = "Journal of the National Cancer Institute",

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T1 - Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts

AU - Brattain, M. G.

AU - Brattain, D. E.

AU - Sarrif, A. M.

AU - McRae, L. J.

AU - Fine, W. D.

AU - Hawkins, J. G.

PY - 1982

Y1 - 1982

N2 - The effects of feeder layers of C3H/10T 1/2 cells on the growth of human and mouse cell lines were tested. Feeder layers increased colony formation by cultured cancerous cells in semisolid medium over controls grown in semisolid medium without feeder layers. Maximal colony formation was also attained at a faster rate when feeder layers were used. Plating efficiency be cancerous cells obtained directly from xenografts was increased threefold to fivefold in tissue culture when feeder cells were present as confluent monolayers.

AB - The effects of feeder layers of C3H/10T 1/2 cells on the growth of human and mouse cell lines were tested. Feeder layers increased colony formation by cultured cancerous cells in semisolid medium over controls grown in semisolid medium without feeder layers. Maximal colony formation was also attained at a faster rate when feeder layers were used. Plating efficiency be cancerous cells obtained directly from xenografts was increased threefold to fivefold in tissue culture when feeder cells were present as confluent monolayers.

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AN - SCOPUS:0019926113

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JO - Journal of the National Cancer Institute

JF - Journal of the National Cancer Institute

SN - 0027-8874

IS - 4

ER -

Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts

Abstract

ASJC Scopus subject areas

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