Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts

M. G. Brattain, D. E. Brattain, A. M. Sarrif, L. J. McRae, W. D. Fine, J. G. Hawkins

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The effects of feeder layers of C3H/10T 1/2 cells on the growth of human and mouse cell lines were tested. Feeder layers increased colony formation by cultured cancerous cells in semisolid medium over controls grown in semisolid medium without feeder layers. Maximal colony formation was also attained at a faster rate when feeder layers were used. Plating efficiency be cancerous cells obtained directly from xenografts was increased threefold to fivefold in tissue culture when feeder cells were present as confluent monolayers.

Original languageEnglish (US)
Pages (from-to)767-771
Number of pages5
JournalJournal of the National Cancer Institute
Volume69
Issue number4
StatePublished - 1982

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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