Enhancement of human intestinal mast cell mediator release in active ulcerative colitis

Charity C. Fox, Audrey J. Lazenby, Wendy C. Moore, John H. Yardley, Theodore M. Bayless, Lawrence M. Lichtenstein

Research output: Contribution to journalArticle

102 Scopus citations

Abstract

To further define the role of mast cells in the idiopathic inflammatory bowel diseases, mediator release from intestinal mast cells derived from actively inflamed and relatively quiescent areas of ulcerative colitis was studied. It was hypothesized that mast cells in the actively diseased segments would indicate involvement in the disease process by releasing a different profile of mediators than cells in uninflamed tissue. Mast cell-containing suspensions derived from matched segments of 12 ulcerative colitis specimens were compared for responsiveness to the mast cell stimulus goat anti-human immunoglobulin E. Supernatants from challenged cells were analyzed for levels of three mast cell mediators, histamine, prostaglandin D2, and the sulfidopeptide leukotriene C. Mast cells from the actively involved areas released significantly greater amounts of histamine, prostaglandin D2, and sulfidopeptide leukotriene. The difference in histamine release was not a result of greater stores of histamine in the active tissue cells, because the total histamine content of the mast cells from the active areas was not significantly greater. The enhanced release of both preformed and newly generated mediators indicates activation of those cells in the course of the disease and points to the mast cell contribution to the inflammatory process in these disorders.

Original languageEnglish (US)
Pages (from-to)119-124
Number of pages6
JournalGastroenterology
Volume99
Issue number1
DOIs
StatePublished - Jul 1990

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Fingerprint Dive into the research topics of 'Enhancement of human intestinal mast cell mediator release in active ulcerative colitis'. Together they form a unique fingerprint.

  • Cite this