Enhancement of metastatic properties of pancreatic cancer cells by MUC1 gene encoding an anti-adhesion molecule

Shuji Satoh, Yuji Hinoda, Toshiaki Hayashi, Michael D. Burdick, Kohzoh Imai, Michael A. Hollingsworth

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

MUC1 mucin expression has been shown to be associated clinicopathologically with metastasis and poor clinical outcome in a variety of tumors. To further investigate this finding experimentally, human pancreatic cancer S2-013 cells overexpressing MUC1 were used for spontaneous metastatic potential in nude mice. It was found that the number of lung metastases of MUC1 transfectants was significantly higher than that of control cells. To analyze the molecular mechanisms that underlie the increased metastatic activity, in vitro adhesion assays were performed. MUC1 mucin expression enhanced in vitro invasiveness and motility of S2-013 cells, and decreased the binding of S2-013 cells to type I collagen, Type IV collagen and laminin. Similar effects were not observed for cells expressing tandem repeat-deleted MUC1 cDNA. Adhesion properties were abolished by benzyl-α-GaINAc treatment, indicating that glycosylation of the extracellular domain of MUC1 was essential for these biological adhesive functions. Our data support the hypothesis that MUC1 expression contributes to the metastatic ability of pancreatic cancer cells. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)507-518
Number of pages12
JournalInternational Journal of Cancer
Volume88
Issue number4
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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