Entrapment of proteins in glycogen-capped and hydrazide-activated supports

Abby J. Jackson; Hai Xuan; David S. Hage

doi:10.1016/j.ab.2010.05.004

Entrapment of proteins in glycogen-capped and hydrazide-activated supports

Abby J. Jackson, Hai Xuan, David S. Hage

Chemistry

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

A method is described for the entrapment of proteins in hydrazide-activated supports using oxidized glycogen as a capping agent. This approach is demonstrated using human serum albumin (HSA) as a model binding agent. After optimization of this method, a protein content of 43 (±1). mg of HSA/g support was obtained for porous silica. The entrapped HSA supports could retain a low-mass drug (S-warfarin) and had activities and equilibrium constants comparable to those for soluble HSA. It was also found that this approach could be used with other proteins and binding agents that had masses between 5.8 and 150. kDa.

Original language	English (US)
Pages (from-to)	106-108
Number of pages	3
Journal	Analytical Biochemistry
Volume	404
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2010.05.004
State	Published - Sep 2010

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.ab.2010.05.004

Cite this

@article{6eb58ac8c4b74fc8aa7472e20569b023,

title = "Entrapment of proteins in glycogen-capped and hydrazide-activated supports",

abstract = "A method is described for the entrapment of proteins in hydrazide-activated supports using oxidized glycogen as a capping agent. This approach is demonstrated using human serum albumin (HSA) as a model binding agent. After optimization of this method, a protein content of 43 (±1). mg of HSA/g support was obtained for porous silica. The entrapped HSA supports could retain a low-mass drug (S-warfarin) and had activities and equilibrium constants comparable to those for soluble HSA. It was also found that this approach could be used with other proteins and binding agents that had masses between 5.8 and 150. kDa.",

author = "Jackson, {Abby J.} and Hai Xuan and Hage, {David S.}",

note = "Funding Information: This work was supported by the National Institutes of Health (NIH) under Grants R01 GM044931 and R01 DK069629 and was performed in facilities renovated under NIH Grant RR015468-01 . ",

year = "2010",

month = sep,

doi = "10.1016/j.ab.2010.05.004",

language = "English (US)",

volume = "404",

pages = "106--108",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Entrapment of proteins in glycogen-capped and hydrazide-activated supports

AU - Jackson, Abby J.

AU - Xuan, Hai

AU - Hage, David S.

N1 - Funding Information: This work was supported by the National Institutes of Health (NIH) under Grants R01 GM044931 and R01 DK069629 and was performed in facilities renovated under NIH Grant RR015468-01 .

PY - 2010/9

Y1 - 2010/9

N2 - A method is described for the entrapment of proteins in hydrazide-activated supports using oxidized glycogen as a capping agent. This approach is demonstrated using human serum albumin (HSA) as a model binding agent. After optimization of this method, a protein content of 43 (±1). mg of HSA/g support was obtained for porous silica. The entrapped HSA supports could retain a low-mass drug (S-warfarin) and had activities and equilibrium constants comparable to those for soluble HSA. It was also found that this approach could be used with other proteins and binding agents that had masses between 5.8 and 150. kDa.

AB - A method is described for the entrapment of proteins in hydrazide-activated supports using oxidized glycogen as a capping agent. This approach is demonstrated using human serum albumin (HSA) as a model binding agent. After optimization of this method, a protein content of 43 (±1). mg of HSA/g support was obtained for porous silica. The entrapped HSA supports could retain a low-mass drug (S-warfarin) and had activities and equilibrium constants comparable to those for soluble HSA. It was also found that this approach could be used with other proteins and binding agents that had masses between 5.8 and 150. kDa.

UR - http://www.scopus.com/inward/record.url?scp=77954083764&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954083764&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2010.05.004

DO - 10.1016/j.ab.2010.05.004

M3 - Article

C2 - 20470745

AN - SCOPUS:77954083764

SN - 0003-2697

VL - 404

SP - 106

EP - 108

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Entrapment of proteins in glycogen-capped and hydrazide-activated supports

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this