TY - JOUR
T1 - Enzyme-linked immunosorbent assay (ELISA) for detecting antibodies in sera of patients with adenovirus infection
AU - Thiele, Geoffrey M.
AU - Okano, Motohiko
AU - Purtilo, David T.
N1 - Funding Information:
We thank Karen Spiegel for preparing this manuscript. Susan Copple, M.T. (ASCP). Robin Cantin, M.T. (ASCP), Mary Anne Johnson, M.T. (ASCP), and Russell D. Mills, M.T. (ASCP) are thanked for their technical assistance. This work was supported in part by PHS CA30196 and CA36727 awarded by the National Cancer Institute, DHHS. the Lymphoproliferative Research Fund, and the State of Nebraska Department of Health, LB506.
PY - 1989/3
Y1 - 1989/3
N2 - The 41 distinct antigenic types of adenoviruses (Ads) are responsible for a broad spectrum of diseases in humans. We have developed an enzyme-linked immunosorbent assay (ELISA) using adenovirus (Ad) infected MRC-5 cells for detecting IgG and IgM antibodies to Ads. Using the ELISA, we detected IgG antibodies in 100% ( 20 20) of sera from normal adults (geometric mean titer, GMT = 1840.8, range = 40-20,480) and IgM antibodies in 3 of 20 sera (15%) with a GMT of 25.1. Our indirect immunofluorescence (IF) technique also detected IgG antibodies in 100%) of these sera (GMT = 248.3, range = 40-5,120) and IgM antibodies in the 3 samples reactive in ELISA (GMT = 20.0, range = < 5-40). In contrast, the complement fixation (CF) test detected antibodies to Ads in only 65% ( 13 20) of these sera (GMT = 10.9, range = < 4-32). Moreover, IgG and IgM responses could not be distinguished using CF. Thus the sensitivity of these three techniques is greatest for ELISA. Additionally, a study of sequential sera from 3 patients with acute Ad infection disclosed seroconversion using all three methods. Both the ELISA and IF techniques permit the detection of transition from IgM to IgG, whereas CF only detects conversion from seronegativity to seropositivity. Finally, preliminary data suggest that the IgM response as measured by ELISA is specific for subgroups or types of Ad. This newly devised ELISA may be useful for detecting Ad infections.
AB - The 41 distinct antigenic types of adenoviruses (Ads) are responsible for a broad spectrum of diseases in humans. We have developed an enzyme-linked immunosorbent assay (ELISA) using adenovirus (Ad) infected MRC-5 cells for detecting IgG and IgM antibodies to Ads. Using the ELISA, we detected IgG antibodies in 100% ( 20 20) of sera from normal adults (geometric mean titer, GMT = 1840.8, range = 40-20,480) and IgM antibodies in 3 of 20 sera (15%) with a GMT of 25.1. Our indirect immunofluorescence (IF) technique also detected IgG antibodies in 100%) of these sera (GMT = 248.3, range = 40-5,120) and IgM antibodies in the 3 samples reactive in ELISA (GMT = 20.0, range = < 5-40). In contrast, the complement fixation (CF) test detected antibodies to Ads in only 65% ( 13 20) of these sera (GMT = 10.9, range = < 4-32). Moreover, IgG and IgM responses could not be distinguished using CF. Thus the sensitivity of these three techniques is greatest for ELISA. Additionally, a study of sequential sera from 3 patients with acute Ad infection disclosed seroconversion using all three methods. Both the ELISA and IF techniques permit the detection of transition from IgM to IgG, whereas CF only detects conversion from seronegativity to seropositivity. Finally, preliminary data suggest that the IgM response as measured by ELISA is specific for subgroups or types of Ad. This newly devised ELISA may be useful for detecting Ad infections.
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U2 - 10.1016/0166-0934(89)90164-X
DO - 10.1016/0166-0934(89)90164-X
M3 - Article
C2 - 2541155
AN - SCOPUS:0024507066
SN - 0166-0934
VL - 23
SP - 321
EP - 332
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 3
ER -