Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed for detection and quantification of serum antibodies to transmissible gastroenteritis virus (TGEV) in swine. Sera from pigs inoculated with cell culture-origin TGEV or gut-origin TGEV were tested for anti-TGEV antibody by ELISA and by serum virus-neutralization test (NT). The ELISA detected antibody 3 days (av) sooner than did the NT when sera from pigs inoculated with cell culture-origin TGEV were tested and 1 day sooner than did the NT when sera from pigs inoculated with gut-origin TGEV were tested. The ELISA appeared to be more sensitive than the NT, since ELISA was more responsive to low-level antibody and ELISA titers exceeded NT titers.
Original language | English (US) |
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Pages (from-to) | 1654-1657 |
Number of pages | 4 |
Journal | American journal of veterinary research |
Volume | 45 |
Issue number | 8 |
State | Published - Aug 1984 |
Externally published | Yes |
ASJC Scopus subject areas
- General Veterinary