Enzyme-linked immunosorbent assay for detection of transmissible gastroenteritis virus antibody in swine sera.

L. D. Nelson, C. L. Kelling

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for detection and quantification of serum antibodies to transmissible gastroenteritis virus (TGEV) in swine. Sera from pigs inoculated with cell culture-origin TGEV or gut-origin TGEV were tested for anti-TGEV antibody by ELISA and by serum virus-neutralization test (NT). The ELISA detected antibody 3 days (av) sooner than did the NT when sera from pigs inoculated with cell culture-origin TGEV were tested and 1 day sooner than did the NT when sera from pigs inoculated with gut-origin TGEV were tested. The ELISA appeared to be more sensitive than the NT, since ELISA was more responsive to low-level antibody and ELISA titers exceeded NT titers.

Original languageEnglish (US)
Pages (from-to)1654-1657
Number of pages4
JournalAmerican journal of veterinary research
Volume45
Issue number8
StatePublished - Aug 1984

ASJC Scopus subject areas

  • veterinary(all)

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