Epidermal growth factor-induced topoisomerase(s). Intracellular translocation and relation to DNA synthesis

Robin Miskimins, W. Keith Miskimins, Harris Bernstein, Nobuyoshi Shimizu

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

We have used epidermal growth factor (EGF) to investigate the relationship between eukaryotic topoisomerases and DNA synthesis. We found that EGF stimulates topoisomerase activity in human fibroblasts and Swiss/3T3 mouse fibroblasts. The first increase is seen in the cytoplasm, followed by increased activity in the nucleus. The nuclear increases correspond to increases in DNA synthesis. A type II topoisomerase is stimulated as indicated by the ATP dependence of the relaxing reaction and by the formation of catenanes. We have also found that the topoisomerase activity in the cytoplasm is sedimentable indicating that it is either membrane-associated or in a supramolecular complex. The stimulation of topoisomerase activity by EGF may represent a key step in the process by which EGF induces DNA synthesis and cell division.

Original languageEnglish (US)
Pages (from-to)53-62
Number of pages10
JournalExperimental Cell Research
Volume146
Issue number1
DOIs
StatePublished - Jun 1983
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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