The authors note that "the primer sequences listed to quantify myb expression by qPCR actually target runx1 instead. We were unable to verify that the correct myb primers were used in the experiment, but two additional independent experiments (Fig. 1C quantification of myb:GFP+ cells and Fig. S2A whole-mount in situ hybridization for myb) validate our conclusion that myb expression is increased in chd7 morphant embryos." The authors note that on page 23634, left column, fourth full paragraph, line 12, "qPCR primers used were the following: Myb forward-5'-CCGACAGAAGCCGGATGA-3' and myb reverse-5'- TGGCACTTCGCCTCAACTG-3'; runx1 forward-5'-CGTCTTCACAAACCCTCCTCAA- 3' and runx1 reverse-5'-GCTTTACTGCTTCATCCGGCT- 3'." should instead appear as "qPCR primers used were the following: Runx1 forward-1-5'-CCGACAGAAGCCGGATGA- 3' and runx1 reverse-1-5'- TGGCACTTCGCCTCA ACTG-3'; runx1 forward-2-5'-CGTCTTCACAAACCCTCCTCAA- 3' and runx1 reverse-2-5' GCTTTACTGCTTCATCCGGCT- 3'." The online version has been corrected.
|Original language||English (US)|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - Sep 28 2021|
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