Erratum: CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation (Proceedings of the National Academy of Sciences of the United States of America (2020) 117 (23626-23635) DOI: 10.1073/pnas.2003228117)

Jingmei Hsu; Hsuan Ting Huang; Chung Tsai Lee; Avik Choudhuri; Nicola K. Wilson; Brian J. Abraham; Victoria Moignard; Iwo Kucinski; Shuqian Yu; R. Katherine Hyde; Joanna Tober; Xiongwei Cai; Yan Li; Yalin Guo; Song Yang; Michael Superdock; Eirini Trompouki; Fernando J. Calero-Nieto; Alireza Ghamari; Jing Jiang; Peng Gao; Long Gao; Vy Nguyen; Anne L. Robertson; Ellen M. Durand; Katie L. Kathrein; Iannis Aifantis; Scott A. Gerber; Wei Tong; Kai Tan; Alan B. Cantor; Yi Zhou; P. Paul Liu; Richard A. Young; Berthold Göttgens; Nancy A. Speck; Leonard I. Zon

doi:10.1073/pnas.2114827118

Erratum: CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation (Proceedings of the National Academy of Sciences of the United States of America (2020) 117 (23626-23635) DOI: 10.1073/pnas.2003228117)

Jingmei Hsu, Hsuan Ting Huang, Chung Tsai Lee, Avik Choudhuri, Nicola K. Wilson, Brian J. Abraham, Victoria Moignard, Iwo Kucinski, Shuqian Yu, R. Katherine Hyde, Joanna Tober, Xiongwei Cai, Yan Li, Yalin Guo, Song Yang, Michael Superdock, Eirini Trompouki, Fernando J. Calero-Nieto, Alireza Ghamari, Jing JiangPeng Gao, Long Gao, Vy Nguyen, Anne L. Robertson, Ellen M. Durand, Katie L. Kathrein, Iannis Aifantis, Scott A. Gerber, Wei Tong, Kai Tan, Alan B. Cantor, Yi Zhou, P. Paul Liu, Richard A. Young, Berthold Göttgens, Nancy A. Speck, Leonard I. Zon

Research output: Contribution to journal › Comment/debate › peer-review

Abstract

The authors note that "the primer sequences listed to quantify myb expression by qPCR actually target runx1 instead. We were unable to verify that the correct myb primers were used in the experiment, but two additional independent experiments (Fig. 1C quantification of myb:GFP+ cells and Fig. S2A whole-mount in situ hybridization for myb) validate our conclusion that myb expression is increased in chd7 morphant embryos." The authors note that on page 23634, left column, fourth full paragraph, line 12, "qPCR primers used were the following: Myb forward-5'-CCGACAGAAGCCGGATGA-3' and myb reverse-5'- TGGCACTTCGCCTCAACTG-3'; runx1 forward-5'-CGTCTTCACAAACCCTCCTCAA- 3' and runx1 reverse-5'-GCTTTACTGCTTCATCCGGCT- 3'." should instead appear as "qPCR primers used were the following: Runx1 forward-1-5'-CCGACAGAAGCCGGATGA- 3' and runx1 reverse-1-5'- TGGCACTTCGCCTCA ACTG-3'; runx1 forward-2-5'-CGTCTTCACAAACCCTCCTCAA- 3' and runx1 reverse-2-5' GCTTTACTGCTTCATCCGGCT- 3'." The online version has been corrected.

Original language	English (US)
Article number	e2114827118
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	118
Issue number	39
DOIs	https://doi.org/10.1073/pnas.2114827118
State	Published - Sep 28 2021

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Hsu, J., Huang, H. T., Lee, C. T., Choudhuri, A., Wilson, N. K., Abraham, B. J., Moignard, V., Kucinski, I., Yu, S., Hyde, R. K., Tober, J., Cai, X., Li, Y., Guo, Y., Yang, S., Superdock, M., Trompouki, E., Calero-Nieto, F. J., Ghamari, A., ... Zon, L. I. (2021). Erratum: CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation (Proceedings of the National Academy of Sciences of the United States of America (2020) 117 (23626-23635) DOI: 10.1073/pnas.2003228117). Proceedings of the National Academy of Sciences of the United States of America, 118(39), Article e2114827118. https://doi.org/10.1073/pnas.2114827118

Erratum: CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation (Proceedings of the National Academy of Sciences of the United States of America (2020) 117 (23626-23635) DOI: 10.1073/pnas.2003228117). / Hsu, Jingmei; Huang, Hsuan Ting; Lee, Chung Tsai et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 118, No. 39, e2114827118, 28.09.2021.

Research output: Contribution to journal › Comment/debate › peer-review

Hsu, J, Huang, HT, Lee, CT, Choudhuri, A, Wilson, NK, Abraham, BJ, Moignard, V, Kucinski, I, Yu, S, Hyde, RK, Tober, J, Cai, X, Li, Y, Guo, Y, Yang, S, Superdock, M, Trompouki, E, Calero-Nieto, FJ, Ghamari, A, Jiang, J, Gao, P, Gao, L, Nguyen, V, Robertson, AL, Durand, EM, Kathrein, KL, Aifantis, I, Gerber, SA, Tong, W, Tan, K, Cantor, AB, Zhou, Y, Liu, PP, Young, RA, Göttgens, B, Speck, NA & Zon, LI 2021, 'Erratum: CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation (Proceedings of the National Academy of Sciences of the United States of America (2020) 117 (23626-23635) DOI: 10.1073/pnas.2003228117)', Proceedings of the National Academy of Sciences of the United States of America, vol. 118, no. 39, e2114827118. https://doi.org/10.1073/pnas.2114827118

Hsu J, Huang HT, Lee CT, Choudhuri A, Wilson NK, Abraham BJ et al. Erratum: CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation (Proceedings of the National Academy of Sciences of the United States of America (2020) 117 (23626-23635) DOI: 10.1073/pnas.2003228117). Proceedings of the National Academy of Sciences of the United States of America. 2021 Sep 28;118(39):e2114827118. doi: 10.1073/pnas.2114827118

Hsu, Jingmei ; Huang, Hsuan Ting ; Lee, Chung Tsai et al. / Erratum : CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation (Proceedings of the National Academy of Sciences of the United States of America (2020) 117 (23626-23635) DOI: 10.1073/pnas.2003228117). In: Proceedings of the National Academy of Sciences of the United States of America. 2021 ; Vol. 118, No. 39.

@article{751d389bedf84752b2778a93a2c832c1,

title = "Erratum: CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation (Proceedings of the National Academy of Sciences of the United States of America (2020) 117 (23626-23635) DOI: 10.1073/pnas.2003228117)",

abstract = "The authors note that {"}the primer sequences listed to quantify myb expression by qPCR actually target runx1 instead. We were unable to verify that the correct myb primers were used in the experiment, but two additional independent experiments (Fig. 1C quantification of myb:GFP+ cells and Fig. S2A whole-mount in situ hybridization for myb) validate our conclusion that myb expression is increased in chd7 morphant embryos.{"} The authors note that on page 23634, left column, fourth full paragraph, line 12, {"}qPCR primers used were the following: Myb forward-5'-CCGACAGAAGCCGGATGA-3' and myb reverse-5'- TGGCACTTCGCCTCAACTG-3'; runx1 forward-5'-CGTCTTCACAAACCCTCCTCAA- 3' and runx1 reverse-5'-GCTTTACTGCTTCATCCGGCT- 3'.{"} should instead appear as {"}qPCR primers used were the following: Runx1 forward-1-5'-CCGACAGAAGCCGGATGA- 3' and runx1 reverse-1-5'- TGGCACTTCGCCTCA ACTG-3'; runx1 forward-2-5'-CGTCTTCACAAACCCTCCTCAA- 3' and runx1 reverse-2-5' GCTTTACTGCTTCATCCGGCT- 3'.{"} The online version has been corrected.",

author = "Jingmei Hsu and Huang, {Hsuan Ting} and Lee, {Chung Tsai} and Avik Choudhuri and Wilson, {Nicola K.} and Abraham, {Brian J.} and Victoria Moignard and Iwo Kucinski and Shuqian Yu and Hyde, {R. Katherine} and Joanna Tober and Xiongwei Cai and Yan Li and Yalin Guo and Song Yang and Michael Superdock and Eirini Trompouki and Calero-Nieto, {Fernando J.} and Alireza Ghamari and Jing Jiang and Peng Gao and Long Gao and Vy Nguyen and Robertson, {Anne L.} and Durand, {Ellen M.} and Kathrein, {Katie L.} and Iannis Aifantis and Gerber, {Scott A.} and Wei Tong and Kai Tan and Cantor, {Alan B.} and Yi Zhou and Liu, {P. Paul} and Young, {Richard A.} and Berthold G{\"o}ttgens and Speck, {Nancy A.} and Zon, {Leonard I.}",

year = "2021",

month = sep,

day = "28",

doi = "10.1073/pnas.2114827118",

language = "English (US)",

volume = "118",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "39",

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TY - JOUR

T1 - Erratum

T2 - CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation (Proceedings of the National Academy of Sciences of the United States of America (2020) 117 (23626-23635) DOI: 10.1073/pnas.2003228117)

AU - Hsu, Jingmei

AU - Huang, Hsuan Ting

AU - Lee, Chung Tsai

AU - Choudhuri, Avik

AU - Wilson, Nicola K.

AU - Abraham, Brian J.

AU - Moignard, Victoria

AU - Kucinski, Iwo

AU - Yu, Shuqian

AU - Hyde, R. Katherine

AU - Tober, Joanna

AU - Cai, Xiongwei

AU - Li, Yan

AU - Guo, Yalin

AU - Yang, Song

AU - Superdock, Michael

AU - Trompouki, Eirini

AU - Calero-Nieto, Fernando J.

AU - Ghamari, Alireza

AU - Jiang, Jing

AU - Gao, Peng

AU - Gao, Long

AU - Nguyen, Vy

AU - Robertson, Anne L.

AU - Durand, Ellen M.

AU - Kathrein, Katie L.

AU - Aifantis, Iannis

AU - Gerber, Scott A.

AU - Tong, Wei

AU - Tan, Kai

AU - Cantor, Alan B.

AU - Zhou, Yi

AU - Liu, P. Paul

AU - Young, Richard A.

AU - Göttgens, Berthold

AU - Speck, Nancy A.

AU - Zon, Leonard I.

PY - 2021/9/28

Y1 - 2021/9/28

N2 - The authors note that "the primer sequences listed to quantify myb expression by qPCR actually target runx1 instead. We were unable to verify that the correct myb primers were used in the experiment, but two additional independent experiments (Fig. 1C quantification of myb:GFP+ cells and Fig. S2A whole-mount in situ hybridization for myb) validate our conclusion that myb expression is increased in chd7 morphant embryos." The authors note that on page 23634, left column, fourth full paragraph, line 12, "qPCR primers used were the following: Myb forward-5'-CCGACAGAAGCCGGATGA-3' and myb reverse-5'- TGGCACTTCGCCTCAACTG-3'; runx1 forward-5'-CGTCTTCACAAACCCTCCTCAA- 3' and runx1 reverse-5'-GCTTTACTGCTTCATCCGGCT- 3'." should instead appear as "qPCR primers used were the following: Runx1 forward-1-5'-CCGACAGAAGCCGGATGA- 3' and runx1 reverse-1-5'- TGGCACTTCGCCTCA ACTG-3'; runx1 forward-2-5'-CGTCTTCACAAACCCTCCTCAA- 3' and runx1 reverse-2-5' GCTTTACTGCTTCATCCGGCT- 3'." The online version has been corrected.

AB - The authors note that "the primer sequences listed to quantify myb expression by qPCR actually target runx1 instead. We were unable to verify that the correct myb primers were used in the experiment, but two additional independent experiments (Fig. 1C quantification of myb:GFP+ cells and Fig. S2A whole-mount in situ hybridization for myb) validate our conclusion that myb expression is increased in chd7 morphant embryos." The authors note that on page 23634, left column, fourth full paragraph, line 12, "qPCR primers used were the following: Myb forward-5'-CCGACAGAAGCCGGATGA-3' and myb reverse-5'- TGGCACTTCGCCTCAACTG-3'; runx1 forward-5'-CGTCTTCACAAACCCTCCTCAA- 3' and runx1 reverse-5'-GCTTTACTGCTTCATCCGGCT- 3'." should instead appear as "qPCR primers used were the following: Runx1 forward-1-5'-CCGACAGAAGCCGGATGA- 3' and runx1 reverse-1-5'- TGGCACTTCGCCTCA ACTG-3'; runx1 forward-2-5'-CGTCTTCACAAACCCTCCTCAA- 3' and runx1 reverse-2-5' GCTTTACTGCTTCATCCGGCT- 3'." The online version has been corrected.

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JO - Proceedings of the National Academy of Sciences of the United States of America

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Erratum: CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation (Proceedings of the National Academy of Sciences of the United States of America (2020) 117 (23626-23635) DOI: 10.1073/pnas.2003228117)

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