Error-tolerant EST database searches by tandem mass spectrometry and multiTag software

Adam J. Liska, Shamil Sunyaev, Ignat N. Shilov, Dan A. Schaeffer, Andrej Shevchenko

Research output: Contribution to journalArticlepeer-review

Abstract

The MultiTag method (Sunyaev et al., Anal. Chem. 2003 15, 1307-1315) employs multiple error-tolerant searches with peptide sequence tags (Mann and Wilm, Anal. Chem. 1994, 66, 4390-4399) for the identification of proteins from organisms with unsequenced genomes. Here we demonstrate that the error-tolerant capabilities of MultiTag increased the number of peptide alignments and improved the confidence of identifications in an EST database. The MultiTag outperformed conventional database searching software that only utilizes stringent matching of tandem mass spectra to nucleotide sequences of ESTs.

Original languageEnglish (US)
Pages (from-to)4118-4122
Number of pages5
JournalProteomics
Volume5
Issue number16
DOIs
StatePublished - Nov 2005
Externally publishedYes

Keywords

  • Database searching
  • Error-tolerant searches
  • EST database
  • Mass spectrometry
  • Protein identification

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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