Establishment and characterization of a new, spontaneously immortalized, pancreatic ductal cell line from the Syrian golden hamster

Toshiyuki Takahashi, Mary P. Moyer, Martin Cano, Qiming J. Wang, Thomas E. Adrian, Charles P. Mountjoy, Warren Sanger, Hiroshi Sugiura, Hiroyuki Katoh, Parviz M. Pour

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Spontaneously immortal pancreatic cell lines are not available. By use of a defined culture medium, such a line (TAKA-1) was established from the Syrian golden hamster. Cytological, cytogenetic, molecular biological, enzymatic and receptor patterns as well as antigenicity were studied and were compared with those of the normal hamster pancreatic ductal cells in vivo. TAKA-1 cells grew exponentially in a monolayer on collagen gel in a defined medium but did not proliferate in soft agar. Ultrastructurally, the cells closely resembled the normal hamster pancreatic ductal cells. Similarities and dissimilarities were found between the normal ductal cells and TAKA-1 cells. Similarities included the presence of cytokeratin, carbonic anhydrase and some tumor-associated antigens. However, unlike the normal ductal cells, TAKA-1 cells expressed blood group A angigen and anti-vimentin, showed affinity to selected lectins, and an abnormality of chromosome 3, which is suggested to be associated with immortality. Moreover, unlike the hamster pancreatic ductal cancer cells but like the normal hamster pancreatic ductal cells, TAKA-1 cells did not have a c-Ki-ras mutation. EGF, TGF-α and secretin, but not CCK or GRP, bound to the TAKA-1 cells. TAKA-1 cells produced TGF-α, and their growth was stimulated by exogenous EGF in serum-free medium. This cell line presents a suitable model for biologic and pathologic study of the hamster pancreatic ductal cells in vitro.

Original languageEnglish (US)
Pages (from-to)163-174
Number of pages12
JournalCell & Tissue Research
Volume282
Issue number1
DOIs
StatePublished - Oct 1 1995
Externally publishedYes

Keywords

  • Cell culture
  • Golden hamster
  • Immortal cell line
  • Karyotype biology
  • Pancreatic ductal cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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    Takahashi, T., Moyer, M. P., Cano, M., Wang, Q. J., Adrian, T. E., Mountjoy, C. P., Sanger, W., Sugiura, H., Katoh, H., & Pour, P. M. (1995). Establishment and characterization of a new, spontaneously immortalized, pancreatic ductal cell line from the Syrian golden hamster. Cell & Tissue Research, 282(1), 163-174. https://doi.org/10.1007/BF00319143