TY - JOUR
T1 - Estimating Bacterial Diversity in Scirtothrips dorsalis (Thysanoptera
T2 - Thripidae) via Next Generation Sequencing
AU - Dickey, Aaron M.
AU - Trease, Andrew J.
AU - Jara-Cavieres, Antonella
AU - Kumar, Vivek
AU - Christenson, Matthew K.
AU - Potluri, Lakshmi Prasad
AU - Morgan, J. Kent
AU - Shatters, Robert G.
AU - McKenzie, Cindy L.
AU - Davis, Paul H.
AU - Osborne, Lance S.
PY - 2014/6
Y1 - 2014/6
N2 - The last 2 decades have produced a better understanding of insect-microbial associations and yielded some important opportunities for insect control. However, most of our knowledge comes from model systems. Thrips (Thysanoptera: Thripidae) have been understudied despite their global importance as invasive species, plant pests and disease vectors. Using a culture and primer independent next-generation sequencing and metagenomics pipeline, we surveyed the bacteria of the globally important pest, Scirtothrips dorsalis Hood. The most abundant bacterial phyla identified were Actinobacteria and Proteobacteria and the most abundant genera were Propionibacterium, Stenotrophomonas, and Pseudomonas. A total of 189 genera of bacteria were identified. The absence of any vertically transferred symbiont taxa commonly found in insects is consistent with other studies suggesting that thrips primarilly acquire resident microbes from their environment. This does not preclude a possible beneficial/intimate association between S. dorsalis and the dominant taxa identified and future work should determine the nature of these associations.
AB - The last 2 decades have produced a better understanding of insect-microbial associations and yielded some important opportunities for insect control. However, most of our knowledge comes from model systems. Thrips (Thysanoptera: Thripidae) have been understudied despite their global importance as invasive species, plant pests and disease vectors. Using a culture and primer independent next-generation sequencing and metagenomics pipeline, we surveyed the bacteria of the globally important pest, Scirtothrips dorsalis Hood. The most abundant bacterial phyla identified were Actinobacteria and Proteobacteria and the most abundant genera were Propionibacterium, Stenotrophomonas, and Pseudomonas. A total of 189 genera of bacteria were identified. The absence of any vertically transferred symbiont taxa commonly found in insects is consistent with other studies suggesting that thrips primarilly acquire resident microbes from their environment. This does not preclude a possible beneficial/intimate association between S. dorsalis and the dominant taxa identified and future work should determine the nature of these associations.
KW - Metagenomics
KW - Next Generation Sequencing
KW - chilli thrips
UR - http://www.scopus.com/inward/record.url?scp=84904106692&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84904106692&partnerID=8YFLogxK
U2 - 10.1896/054.097.0204
DO - 10.1896/054.097.0204
M3 - Article
AN - SCOPUS:84904106692
VL - 97
SP - 362
EP - 366
JO - Florida Entomologist
JF - Florida Entomologist
SN - 0015-4040
IS - 2
ER -