Estimating the probability that a genomic library contains a specific DNA sequence

C. O. Gardner; K. M. Eskridge; H. T. Zhang; D. P. Weeks

doi:10.2307/1400418

Estimating the probability that a genomic library contains a specific DNA sequence

C. O. Gardner, K. M. Eskridge, H. T. Zhang, D. P. Weeks

Statistics

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A genomic library generally contains a random collection of DNA fragments from one organism individually cloned into cells of a host organism using plasmid or viral vectors. For a genomic library to be reliable, it is crucial that the library have a high probability of containing a complete copy of any particular gene being studied. In this article, we compare several different methods of estimating this probability based on data from a genomic library of Chlamydomonas reinhardtii DNA. Results suggest that a method based on estimated insert size will generally be preferred because of its precision and cost effectiveness relative to other methods.

Original language	English (US)
Pages (from-to)	1-8
Number of pages	8
Journal	Journal of Agricultural, Biological, and Environmental Statistics
Volume	4
Issue number	1
DOIs	https://doi.org/10.2307/1400418
State	Published - Mar 1999

Keywords

Binomial proportion
Gene library
Reliability

ASJC Scopus subject areas

Statistics and Probability
Agricultural and Biological Sciences (miscellaneous)
Environmental Science(all)
Agricultural and Biological Sciences(all)
Statistics, Probability and Uncertainty
Applied Mathematics

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AU - Zhang, H. T.

AU - Weeks, D. P.

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AB - A genomic library generally contains a random collection of DNA fragments from one organism individually cloned into cells of a host organism using plasmid or viral vectors. For a genomic library to be reliable, it is crucial that the library have a high probability of containing a complete copy of any particular gene being studied. In this article, we compare several different methods of estimating this probability based on data from a genomic library of Chlamydomonas reinhardtii DNA. Results suggest that a method based on estimated insert size will generally be preferred because of its precision and cost effectiveness relative to other methods.

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KW - Reliability

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Estimating the probability that a genomic library contains a specific DNA sequence

Abstract

Keywords

ASJC Scopus subject areas

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