Abstract
A genomic library generally contains a random collection of DNA fragments from one organism individually cloned into cells of a host organism using plasmid or viral vectors. For a genomic library to be reliable, it is crucial that the library have a high probability of containing a complete copy of any particular gene being studied. In this article, we compare several different methods of estimating this probability based on data from a genomic library of Chlamydomonas reinhardtii DNA. Results suggest that a method based on estimated insert size will generally be preferred because of its precision and cost effectiveness relative to other methods.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-8 |
| Number of pages | 8 |
| Journal | Journal of Agricultural, Biological, and Environmental Statistics |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1999 |
Keywords
- Binomial proportion
- Gene library
- Reliability
ASJC Scopus subject areas
- Statistics and Probability
- Agricultural and Biological Sciences (miscellaneous)
- Environmental Science(all)
- Agricultural and Biological Sciences(all)
- Statistics, Probability and Uncertainty
- Applied Mathematics
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Cite this
Gardner, C. O., Eskridge, K. M., Zhang, H. T., & Weeks, D. P. (1999). Estimating the probability that a genomic library contains a specific DNA sequence. Journal of Agricultural, Biological, and Environmental Statistics, 4(1), 1-8. https://doi.org/10.2307/1400418