TY - JOUR
T1 - Evaluating the antimicrobial and anti-biofilm activity of three synthetic antimicrobial Citropin analogs and their ability to fight against Staphylococcus aureus and Staph ylococcus pseudinter medius
AU - Salvado, Matheus Gomes
AU - André, Lialyz Soares Pereira
AU - Pereira, Renata Freire Alves
AU - Pinheiro, Felipe Ramos
AU - das Chagas Barbosa, Beatriz
AU - Scaffo, Julia Chaves
AU - Pereira, Aramis José
AU - Arakaki, Daniela Granja
AU - Xing, Huihua
AU - de Oliveira, Kelly Mari Pires
AU - de Andrade dos Santos, João Víctor
AU - Sachs, Daniela
AU - Aguiar-Alves, Fabio
AU - Conda-Sheridan, Martin
AU - Penna, Bruno
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/6
Y1 - 2024/6
N2 - Aims: We de v eloped three ne w analogs of the antimicrobial peptide (AMP) Citropin 1.1: DAN-1-13, AJP-1-1, and HHX-2-28, and tested their potential antimicrobial and antibiofilm activities against Staphylococcus aureus and S. pseudintermedius . Potential cytotoxic or hemolytic effects were determined using cultured human keratinocytes and erythrocytes to determine their safety. Methods and results: To assess the antimicrobial activity of each compound, minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined against methicillin-resistant and methicillin-susceptible strains of S. aureus and S. pseudintermedius . A ctivity against ne wly f ormed and mature biofilms w as determined in two clinical isolates using spectrophotometry and scanning electron microscopy (SEM). All three compounds exhibited antimicrobial and bactericidal activity against all studied S. aureus and S. pseudintermedius strains, with MICs ranging from 4-32 μg ml -1 and MBCs ranging from 8-128 μg ml -1 . Subinhibitory concentrations of all compounds also sho w ed ant-biofilm activity in the two tested isolates. All compounds exhibited limited cytotoxic and hemolytic activity. Conclusions: No v el analogs of Citropin 1.1 exhibit antimicrobial and bactericidal activities against S. aureus and S. pseudintermedius isolates and inhibit the biofilm formation of these bacteria. Impact Statement Antimicrobial peptides (AMPs) constitute an attractive option for combating resistance to conventional antibiotics. This study demonstrated the antimicrobial and antibiofilm activity of no v el AMPs, analogous to Citropin 1.1, against Staphylococcus aureus and S. pseudintermedius . These results underscore the therapeutic potential of Citropin 1.1 analogs to fight staphylococcal infections.
AB - Aims: We de v eloped three ne w analogs of the antimicrobial peptide (AMP) Citropin 1.1: DAN-1-13, AJP-1-1, and HHX-2-28, and tested their potential antimicrobial and antibiofilm activities against Staphylococcus aureus and S. pseudintermedius . Potential cytotoxic or hemolytic effects were determined using cultured human keratinocytes and erythrocytes to determine their safety. Methods and results: To assess the antimicrobial activity of each compound, minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined against methicillin-resistant and methicillin-susceptible strains of S. aureus and S. pseudintermedius . A ctivity against ne wly f ormed and mature biofilms w as determined in two clinical isolates using spectrophotometry and scanning electron microscopy (SEM). All three compounds exhibited antimicrobial and bactericidal activity against all studied S. aureus and S. pseudintermedius strains, with MICs ranging from 4-32 μg ml -1 and MBCs ranging from 8-128 μg ml -1 . Subinhibitory concentrations of all compounds also sho w ed ant-biofilm activity in the two tested isolates. All compounds exhibited limited cytotoxic and hemolytic activity. Conclusions: No v el analogs of Citropin 1.1 exhibit antimicrobial and bactericidal activities against S. aureus and S. pseudintermedius isolates and inhibit the biofilm formation of these bacteria. Impact Statement Antimicrobial peptides (AMPs) constitute an attractive option for combating resistance to conventional antibiotics. This study demonstrated the antimicrobial and antibiofilm activity of no v el AMPs, analogous to Citropin 1.1, against Staphylococcus aureus and S. pseudintermedius . These results underscore the therapeutic potential of Citropin 1.1 analogs to fight staphylococcal infections.
KW - Staphylococcus spp. antibiofilm activity
KW - synthetic antimicrobial peptides
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U2 - 10.1093/jambio/lxae127
DO - 10.1093/jambio/lxae127
M3 - Article
C2 - 38772746
AN - SCOPUS:85196173999
SN - 1364-5072
VL - 135
JO - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
IS - 6
M1 - lxae127
ER -