Evaluating the antimicrobial and anti-biofilm activity of three synthetic antimicrobial Citropin analogs and their ability to fight against Staphylococcus aureus and Staph ylococcus pseudinter medius

Matheus Gomes Salvado, Lialyz Soares Pereira André, Renata Freire Alves Pereira, Felipe Ramos Pinheiro, Beatriz das Chagas Barbosa, Julia Chaves Scaffo, Aramis José Pereira, Daniela Granja Arakaki, Huihua Xing, Kelly Mari Pires de Oliveira, João Víctor de Andrade dos Santos, Daniela Sachs, Fabio Aguiar-Alves, Martin Conda-Sheridan, Bruno Penna

Research output: Contribution to journalArticlepeer-review

Abstract

Aims: We de v eloped three ne w analogs of the antimicrobial peptide (AMP) Citropin 1.1: DAN-1-13, AJP-1-1, and HHX-2-28, and tested their potential antimicrobial and antibiofilm activities against Staphylococcus aureus and S. pseudintermedius . Potential cytotoxic or hemolytic effects were determined using cultured human keratinocytes and erythrocytes to determine their safety. Methods and results: To assess the antimicrobial activity of each compound, minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined against methicillin-resistant and methicillin-susceptible strains of S. aureus and S. pseudintermedius . A ctivity against ne wly f ormed and mature biofilms w as determined in two clinical isolates using spectrophotometry and scanning electron microscopy (SEM). All three compounds exhibited antimicrobial and bactericidal activity against all studied S. aureus and S. pseudintermedius strains, with MICs ranging from 4-32 μg ml -1 and MBCs ranging from 8-128 μg ml -1 . Subinhibitory concentrations of all compounds also sho w ed ant-biofilm activity in the two tested isolates. All compounds exhibited limited cytotoxic and hemolytic activity. Conclusions: No v el analogs of Citropin 1.1 exhibit antimicrobial and bactericidal activities against S. aureus and S. pseudintermedius isolates and inhibit the biofilm formation of these bacteria. Impact Statement Antimicrobial peptides (AMPs) constitute an attractive option for combating resistance to conventional antibiotics. This study demonstrated the antimicrobial and antibiofilm activity of no v el AMPs, analogous to Citropin 1.1, against Staphylococcus aureus and S. pseudintermedius . These results underscore the therapeutic potential of Citropin 1.1 analogs to fight staphylococcal infections.

Original languageEnglish (US)
Article numberlxae127
JournalJournal of Applied Microbiology
Volume135
Issue number6
DOIs
StatePublished - Jun 2024

Keywords

  • Staphylococcus spp. antibiofilm activity
  • synthetic antimicrobial peptides

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'Evaluating the antimicrobial and anti-biofilm activity of three synthetic antimicrobial Citropin analogs and their ability to fight against Staphylococcus aureus and Staph ylococcus pseudinter medius'. Together they form a unique fingerprint.

Cite this