Evaluating the metabolic alterations in pancreatic cancer

Aneesha Dasgupta, Surendra K. Shukla, Venugopal Gunda, Ryan J. King, Pankaj K. Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations


Metabolic reprograming is an established hallmark of cancer cells. Pancreatic cancer cells, by virtue of the underlying oncogenic drivers, demonstrate metabolic reprograming to sustain growth, invasiveness, and therapy resistance. The increased demands of the growing tumor cells alter the metabolic and signaling pathways to meet the growing nutrient requirements. Investigating the metabolic vulnerabilities of tumor cells can help in developing effective therapeutics to target pancreatic cancer. In this chapter, we explain in detail the methods to evaluate the metabolic changes occurring in the tumor. This includes the glucose/glutamine uptake assays and the measurement of reactive oxygen species, extracellular acidification rate, and oxygen consumption rate in the tumor cells. All these physiological assays help in understanding the metabolic nature of the tumor.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages8
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Cancer metabolism
  • ECAR
  • Glucose uptake
  • Glutamine uptake
  • OCR
  • Pancreatic cancer
  • Reactive oxygen species

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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