Evaluating the metabolic impact of hypoxia on pancreatic cancer cells

Divya Murthy, Enza Vernucci, Gennifer Goode, Jaime Abrego, Pankaj K. Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Hypoxia is frequently observed in human cancers and induces global metabolic reprogramming that includes an increase in glucose uptake and glycolysis, alterations in NAD(P)H/NAD(P)+ and intracellular ATP levels, and increased utilization of glutamine as the major precursor for fatty acid synthesis. In this chapter, we describe in detail various physiological assays that have been adopted to study the metabolic shift propagated by exposure to hypoxic conditions in pancreatic cell culture model that includes glucose uptake, glutamine uptake, and lactate release by pancreatic cancer cell lines. We have also elaborated the assays to evaluate the ratio of NAD(P)H/NAD(P)+ and intracellular ATP estimation using the commercially available kit to assess the metabolic state of cancer cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages81-93
Number of pages13
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1742
ISSN (Print)1064-3745

Keywords

  • ATP estimation
  • Cancer metabolism
  • Glucose uptake
  • Glutamine uptake
  • HIF
  • Hypoxia
  • Lactate release
  • NADPH/NADP ratio
  • Pancreatic cancer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Murthy, D., Vernucci, E., Goode, G., Abrego, J., & Singh, P. K. (2018). Evaluating the metabolic impact of hypoxia on pancreatic cancer cells. In Methods in Molecular Biology (pp. 81-93). (Methods in Molecular Biology; Vol. 1742). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7665-2_8