Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples

James L. Bono, James E. Keen, Laura C. Miller, James M. Fox, Carol G. Chitko-McKown, Michael P. Heaton, William W. Laegreid

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g-1 in artificially inoculated bovine feces following enrichment.

Original languageEnglish (US)
Pages (from-to)1855-1857
Number of pages3
JournalApplied and environmental microbiology
Volume70
Issue number3
DOIs
StatePublished - Mar 2004

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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