Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples

James L. Bono; James E. Keen; Laura C. Miller; James M. Fox; Carol G. Chitko-McKown; Michael P. Heaton; William W. Laegreid

doi:10.1128/AEM.70.3.1855-1857.2004

Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples

James L. Bono, James E. Keen, Laura C. Miller, James M. Fox, Carol G. Chitko-McKown, Michael P. Heaton, William W. Laegreid

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g^-1 in artificially inoculated bovine feces following enrichment.

Original language	English (US)
Pages (from-to)	1855-1857
Number of pages	3
Journal	Applied and environmental microbiology
Volume	70
Issue number	3
DOIs	https://doi.org/10.1128/AEM.70.3.1855-1857.2004
State	Published - Mar 2004
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Ecology
Applied Microbiology and Biotechnology

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10.1128/AEM.70.3.1855-1857.2004

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title = "Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples",

abstract = "A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g-1 in artificially inoculated bovine feces following enrichment.",

author = "Bono, {James L.} and Keen, {James E.} and Miller, {Laura C.} and Fox, {James M.} and Chitko-McKown, {Carol G.} and Heaton, {Michael P.} and Laegreid, {William W.}",

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AU - Heaton, Michael P.

AU - Laegreid, William W.

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Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples

Abstract

ASJC Scopus subject areas

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