A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g-1 in artificially inoculated bovine feces following enrichment.
ASJC Scopus subject areas
- Food Science
- Applied Microbiology and Biotechnology