Evaluation of an ELISA for detection of ovine progressive pneumonia antibodies using a recombinant transmembrane envelope protein

Jimmy Kwang, Jim Keen, Randall C. Cutlip, E. Travis Littledike

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed using a recombinant protein corresponding to the N'-terminal hydrophilic region of transmembrane glycoprotein (TM) of ovine lentivirus. This assay reproducibly detected antibodies in sera from 207 of 212 ovine progressive pneumonia (OPP) virus-infected sheep, and the recombinant TM ELISA accurately identified 26% (35 vs. 9) more seropositive samples than did the agar gel immunodiffusion test when applied to 100 sera from an infected flock. This assay also yielded no false-positive results in 14 true negative sera. Results of these experiments were further confirmed by the recombinant TM and recombinant p25 Western blot assay. A single recombinant TM antigen, as the coating antigen in ELISA, can be used successfully for the detection of OPP virus-infected animals and can improve the sensitivity and specificity for OPP diagnosis.

Original languageEnglish (US)
Pages (from-to)189-193
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume5
Issue number2
DOIs
StatePublished - Apr 1993

ASJC Scopus subject areas

  • veterinary(all)

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