Evaluation of primers for PCR amplification of RNA polymerase gene sequences of foot-and-mouth disease virus

B. Pattnaik, A. Sanyal, M. George, C. Tosh, D. Hemadri, R. Venkataramanan

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Eight oligonucleotide primers in 7 different combinations were used to amplify 3D gene sequences of foot-and-mouth disease virus (FMDV) by reverse transcription-polymerase chain reaction (RT-PCR). Six of the primers were designed at this laboratory. All the primer combinations could specifically amplify 3D gene sequences of FMDV serotypes O, A, and C. The largest fragment amplified was of 1393 bp and the smallest was of 208 bp in size. The 1393 bp fragment included sequences from the preceeding P18 region of FMDV genome. The second largest fragment of 734 bp included sequences from the 3'-extracistronic region of viral genome. The remaining fragments were amplified either from the 3'- or 5'-half of the 3D gene. Specific amplification of the entire 3D gene in fragments of different size showed sequence conservation in the 3D genomic region of FMDV and usefulness of the primers reported in detecting inapparent or persistent FMDV infection in susceptible animals by RT-PCR.

Original languageEnglish (US)
Pages (from-to)333-336
Number of pages4
JournalActa Virologica
Volume41
Issue number6
StatePublished - 1997

Keywords

  • 3D gene
  • Foot-and-mouth disease virus
  • Persistent infection
  • Reverse transcription-polymerase chain reaction

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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