Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum.

P. J. Blackall; T. Yamaguchi; Y. Iritani; D. G. Rogers

doi:10.2307/1591374

Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum.

P. J. Blackall, T. Yamaguchi, Y. Iritani, D. G. Rogers

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Two monoclonal antibodies (MAbs) were evaluated for their ability to serotype 108 isolates of Haemophilus paragallinarum. One MAb (E5C12D10) was raised against a Page serovar A strain and the other (F2E6) against a Page serovar C strain. In both dot blot and hemagglutination-inhibition tests, MAb E5C12D10 recognized the type strains of Page serovar A and Kume serovars A-1, A-2, A-3, and A-4. MAb F2E6 recognized the type strains of Page serovar C and Kume serovars C-1, C-2, and C-3. Neither antibody recognized the type strains of Page serovar B or Kume serovars B-1 and C-4. When evaluated with 97 field isolates in a dot blot test, the MAbs serotyped 81 isolates, which was better than agglutinin typing by the Page scheme (69 isolates serotyped). The field isolates that did not react with the MAbs were either Page serovar B/Kume serovar B-1 (three isolates), Page serovar C/Kume serovar C-4 (12 isolates), or nontypable by either the Page or Kume scheme (one isolate).

Original language	English (US)
Pages (from-to)	861-864
Number of pages	4
Journal	Avian diseases
Volume	34
Issue number	4
DOIs	https://doi.org/10.2307/1591374
State	Published - 1990

ASJC Scopus subject areas

Food Animals
Animal Science and Zoology
Immunology and Microbiology(all)

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title = "Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum.",

abstract = "Two monoclonal antibodies (MAbs) were evaluated for their ability to serotype 108 isolates of Haemophilus paragallinarum. One MAb (E5C12D10) was raised against a Page serovar A strain and the other (F2E6) against a Page serovar C strain. In both dot blot and hemagglutination-inhibition tests, MAb E5C12D10 recognized the type strains of Page serovar A and Kume serovars A-1, A-2, A-3, and A-4. MAb F2E6 recognized the type strains of Page serovar C and Kume serovars C-1, C-2, and C-3. Neither antibody recognized the type strains of Page serovar B or Kume serovars B-1 and C-4. When evaluated with 97 field isolates in a dot blot test, the MAbs serotyped 81 isolates, which was better than agglutinin typing by the Page scheme (69 isolates serotyped). The field isolates that did not react with the MAbs were either Page serovar B/Kume serovar B-1 (three isolates), Page serovar C/Kume serovar C-4 (12 isolates), or nontypable by either the Page or Kume scheme (one isolate).",

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Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum.

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