TY - JOUR
T1 - Evidence for a qualitative hierarchy within the Hoechst-33342 'side population' (SP) of murine bone marrow cells
AU - Robinson, S. N.
AU - Seina, S. M.
AU - Gohr, J. C.
AU - Kuszynski, C. A.
AU - Sharp, J. G.
N1 - Funding Information:
We gratefully acknowledge Dr John D Jackson, Department of Pathology and Microbiology, UNMC, for his advice with the preparation of this paper; Linda Wilkie, Cell Processing Facility, UNMC, for her assistance and advice with FACS analysis, Craig Boyer, Department of Genetics, Cell Biology and Anatomy, UNMC, for his technical assistance and Dr Elizabeth J Shpall, Department of Blood and Bone Marrow Transplantation, The University of Texas MD Anderson Cancer Center, Houston, Texas, for her encouragement and support. Research supported by the Dean’s Office of the College of Medicine, UNMC and a Nebraska Research Initiative award.
PY - 2005/4
Y1 - 2005/4
N2 - In vitro cobblestone area (CA)-forming cell (CAFC) and in vivo (short-term and competitive repopulation) assays demonstrate that a qualitative hierarchy exists within the Hoechst-33342-defined side population (SP) in murine bone marrow (BM). Consistent with and extending previous studies, we demonstrate that (i) hematopoietic activity found in whole BM (WBM) is concentrated within the SP, rather than the non-SP (NSP); and (ii) within the SP, those cells that more strongly efflux the dye (lower SP, LSP) are qualitatively different from those that less strongly efflux the dye (upper SP, USP). Qualitative differences are highlighted by evidence that (i) CA derived from LSP CAFC persist in culture significantly longer than CA derived from USP CAFC; (ii) short-term, multilineage repopulation of lethally irradiated mice by LSP cells is more rapid than that in mice receiving USP, NSP, whole SP (WSP), or WBM cells and (iii) LSP cells out-compete USP cells in the multilineage hematopoietic repopulation of lethally irradiated recipients. These data suggest that LSP cells are of higher quality than USP cells and potentially provide a means by which qualitative changes in primitive hematopoietic progenitors occurring naturally with aging, or clinically as a consequence of therapeutic manipulation, can be assessed.
AB - In vitro cobblestone area (CA)-forming cell (CAFC) and in vivo (short-term and competitive repopulation) assays demonstrate that a qualitative hierarchy exists within the Hoechst-33342-defined side population (SP) in murine bone marrow (BM). Consistent with and extending previous studies, we demonstrate that (i) hematopoietic activity found in whole BM (WBM) is concentrated within the SP, rather than the non-SP (NSP); and (ii) within the SP, those cells that more strongly efflux the dye (lower SP, LSP) are qualitatively different from those that less strongly efflux the dye (upper SP, USP). Qualitative differences are highlighted by evidence that (i) CA derived from LSP CAFC persist in culture significantly longer than CA derived from USP CAFC; (ii) short-term, multilineage repopulation of lethally irradiated mice by LSP cells is more rapid than that in mice receiving USP, NSP, whole SP (WSP), or WBM cells and (iii) LSP cells out-compete USP cells in the multilineage hematopoietic repopulation of lethally irradiated recipients. These data suggest that LSP cells are of higher quality than USP cells and potentially provide a means by which qualitative changes in primitive hematopoietic progenitors occurring naturally with aging, or clinically as a consequence of therapeutic manipulation, can be assessed.
KW - CAFC assay
KW - Competitive repopulation
KW - Hematopoietic reconstitution
KW - Primitive hematopoietic progenitor cell
KW - Qualitative hierarchy
KW - Side population (SP)
UR - http://www.scopus.com/inward/record.url?scp=18144400384&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18144400384&partnerID=8YFLogxK
U2 - 10.1038/sj.bmt.1704881
DO - 10.1038/sj.bmt.1704881
M3 - Article
C2 - 15750609
AN - SCOPUS:18144400384
SN - 0268-3369
VL - 35
SP - 807
EP - 818
JO - Bone marrow transplantation
JF - Bone marrow transplantation
IS - 8
ER -