Evidence for a single butyrylcholinesterase gene in individuals carrying the C5 plasma cholinesterase variant (CHE2)

Patrick Masson; Arnaud Chatonnet; Oksana Lockridge

doi:10.1016/0014-5793(90)80167-H

Evidence for a single butyrylcholinesterase gene in individuals carrying the C₅ plasma cholinesterase variant (CHE2)

Patrick Masson, Arnaud Chatonnet, Oksana Lockridge

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

DNA of 3 unrelated individuals carrying the human plasma butyrylcholinesterase C₅ variant (CHE2) was isolated from white blood cells. Southern blot patterns of DNA restriction fragments probed with each of the 4 butyrylcholinesterase exons provided evidence that the production of C₅ is not directed by a second butyrylcholinesterase gene. This finding supports the suggestion that the C₅ variant is a hybrid enzyme resulting from the association of butyrylcholinesterase subunits with a non-cholinesterase protein.

Original language	English (US)
Pages (from-to)	115-118
Number of pages	4
Journal	FEBS Letters
Volume	262
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(90)80167-H
State	Published - Mar 12 1990
Externally published	Yes

Keywords

(Human plasma)
Butyrylcholinesterase
CHE2
Southern blot analysis

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(90)80167-H

Cite this

@article{24a9aa8ac45948df820b2615083f140a,

title = "Evidence for a single butyrylcholinesterase gene in individuals carrying the C5 plasma cholinesterase variant (CHE2)",

abstract = "DNA of 3 unrelated individuals carrying the human plasma butyrylcholinesterase C5 variant (CHE2) was isolated from white blood cells. Southern blot patterns of DNA restriction fragments probed with each of the 4 butyrylcholinesterase exons provided evidence that the production of C5 is not directed by a second butyrylcholinesterase gene. This finding supports the suggestion that the C5 variant is a hybrid enzyme resulting from the association of butyrylcholinesterase subunits with a non-cholinesterase protein.",

keywords = "(Human plasma), Butyrylcholinesterase, CHE2, Southern blot analysis",

author = "Patrick Masson and Arnaud Chatonnet and Oksana Lockridge",

note = "Funding Information: AcknowledgementWs:e would like to thank Mrs M.T. Fromentf or her technicala ssistancea nd Mr S. BtguCa nd the staff of the Centre Regionald e TransfusionS anguined e Lyon for supplyingb lood from donors carryingt he CS cholinesterasvea riant.The DNA work was carried out in Dr Bert N. La Du{\textquoteright}s laboratory (Dept. of Pharmacology,U niversityo f Michigan, Ann Arbor); the authorsa re indebted to Dr La Du for his support and thank him for fruitful discussionsT. his work was supportedb y US Army MedicalR esearch and DevelopmenCt ommandC ontractD AMD17-86-C-6037to O.L. and NIH Grant GM27028-10to Bert N. La Du.",

year = "1990",

month = mar,

day = "12",

doi = "10.1016/0014-5793(90)80167-H",

language = "English (US)",

volume = "262",

pages = "115--118",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Evidence for a single butyrylcholinesterase gene in individuals carrying the C5 plasma cholinesterase variant (CHE2)

AU - Masson, Patrick

AU - Chatonnet, Arnaud

AU - Lockridge, Oksana

N1 - Funding Information: AcknowledgementWs:e would like to thank Mrs M.T. Fromentf or her technicala ssistancea nd Mr S. BtguCa nd the staff of the Centre Regionald e TransfusionS anguined e Lyon for supplyingb lood from donors carryingt he CS cholinesterasvea riant.The DNA work was carried out in Dr Bert N. La Du’s laboratory (Dept. of Pharmacology,U niversityo f Michigan, Ann Arbor); the authorsa re indebted to Dr La Du for his support and thank him for fruitful discussionsT. his work was supportedb y US Army MedicalR esearch and DevelopmenCt ommandC ontractD AMD17-86-C-6037to O.L. and NIH Grant GM27028-10to Bert N. La Du.

PY - 1990/3/12

Y1 - 1990/3/12

N2 - DNA of 3 unrelated individuals carrying the human plasma butyrylcholinesterase C5 variant (CHE2) was isolated from white blood cells. Southern blot patterns of DNA restriction fragments probed with each of the 4 butyrylcholinesterase exons provided evidence that the production of C5 is not directed by a second butyrylcholinesterase gene. This finding supports the suggestion that the C5 variant is a hybrid enzyme resulting from the association of butyrylcholinesterase subunits with a non-cholinesterase protein.

AB - DNA of 3 unrelated individuals carrying the human plasma butyrylcholinesterase C5 variant (CHE2) was isolated from white blood cells. Southern blot patterns of DNA restriction fragments probed with each of the 4 butyrylcholinesterase exons provided evidence that the production of C5 is not directed by a second butyrylcholinesterase gene. This finding supports the suggestion that the C5 variant is a hybrid enzyme resulting from the association of butyrylcholinesterase subunits with a non-cholinesterase protein.

KW - (Human plasma)

KW - Butyrylcholinesterase

KW - CHE2

KW - Southern blot analysis

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