Evidence for nonregulatory trehalase activity in Dictyostelium discoideum

Jyothi Gupta, Steven D. Harris, David A. Cotter

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

An in vitro activation treatment, stimulatory to the regulatory cytoplasmic trehalase of Saccharomyces cerevisiae, had no effect on the lysosomal trehalase of Dictyostelium discoideum. Concentrations of cAMP that produced a 19 to 22-fold increase in trehalase activity in S. cerevisiae extracts did not stimulate trehalase activity in D. discoideum extracts.. cGMP and 5′-AMP were also not effective in activating the enzyme. Dictyostelium discoideum trehalase exhibits characteristics typical of nonregulatory trehalases, in agreement with its lysosomal localization. The data are consistent with the hypothesis that changes in compartmentation regulate trehalose mobilization in D. discoideum.

Original languageEnglish (US)
Pages (from-to)101-104
Number of pages4
JournalCurrent Microbiology
Volume16
Issue number2
DOIs
StatePublished - Mar 1987

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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